A cellular response protein induced during HSV-1 infection inhibits viral replication by interacting with ATF5.

Abstract

Studies of herpes simplex virus type 1 (HSV-1) infection have shown that many known and unknown cellular molecules involved in viral proliferation are up-regulated following HSV-1 infection. In this study, using two-dimensional polyacrylamide gel electrophoresis, we found that the expression of the HSV-1 infection response repressive protein (HIRRP, GI 16552881) was up-regulated in human L02 cells infected with HSV-1. HIRRP, an unknown protein, was initially localized in the cytoplasm and then translocated into the nucleus of HSV-1-infected cells. Further analysis showed that HIRRP represses HSV-1 proliferation by inhibiting transcription of the viral genome by interacting with the cellular transcription factor, ATF5, via its N-terminal domain. ATF5 represses the transcription of many host genes but can also act as an activator of genes containing a specific motif. We found that ATF5 promotes the proliferation of HSV-1 via a potential mechanism by which ATF5 enhances the transcription of viral genes during the course of an HSV-1 infection; HIRRP then induces feedback repression of this transcription by interacting with ATF5.

• Authors: LianQiu Wu, XueMei Zhang, YanChun Che, Ying Zhang, SongQing Tang, Yun Liao, RuiXiong Na, Xianglin Xiong, LongDing Liu, QiHan Li
• Journal: Science China. Life sciences (Sci China Life Sci) Vol. 56 Issue 12 Pg. 1124-33 (Dec 2013) ISSN: 1869-1889 [Electronic] China
• PMID: 24302293 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• ATF5 protein, human
• Activating Transcription Factors
• Carrier Proteins
• DNA, Viral
• HSV-1 infection response repressive protein, human
• Viral Proteins

Topics

• Activating Transcription Factors (chemistry, genetics, physiology)
• Animals
• Base Sequence
• Carrier Proteins (chemistry, genetics, physiology)
• Cell Line
• Cell Nucleus (metabolism)
• Chlorocebus aethiops
• Cytoplasm (metabolism)
• DNA, Viral (genetics, metabolism)
• Electrophoresis, Gel, Two-Dimensional
• Gene Knockdown Techniques
• Genome, Viral
• HEK293 Cells
• HeLa Cells
• Herpesvirus 1, Human (genetics, pathogenicity, physiology)
• Host-Pathogen Interactions (genetics, physiology)
• Humans
• Molecular Sequence Data
• Protein Interaction Domains and Motifs
• Up-Regulation
• Vero Cells
• Viral Proteins (chemistry, genetics, physiology)
• Virus Replication (genetics, physiology)