[Preparation and characterization of antisera specific to various polypeptide domains corresponding to the v-ets oncogene of the avian leukemia virus E26].

Abstract

We prepared antisera to three distinct portions of the v-ets oncogene of the avian leukemia virus E26. An antiserum directed against the middle v-ets-encoded domain identifies in different chicken cell lines and normal tissues a c-ets-encoded protein of Mr 54,000 (P54c-ets) and three proteins of Mr 60,000 62,000 and 64,000 partially related to P54c-ets. Antisera directed against the aminoterminal v-ets-encoded domain failed to precipitate P54c-ets or P60/P64. Thus, the E26 specific v-ets oncogene displays a complex structure that includes several distinct portions, the genetic origin of which could be different.

• Authors: A Gegonne, P Pognonec, D Leprince, D Dernis, E Remaut, D Stehelin, J Ghysdael
• Journal: Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie (C R Acad Sci III) Vol. 303 Issue 7 Pg. 253-6 ( 1986) ISSN: 0764-4469 [Print] France
• Vernacular Title: Préparation et caractérisation d'antisera specifiques de différents domaines polypeptidiques correspondant à l'oncogène v-ets du virus aviaire de leucémies aiguës E26.
• PMID: 2429743 (Publication Type: English Abstract, Journal Article)

Chemical References

• Antigen-Antibody Complex
• Epitopes
• Immune Sera
• Viral Proteins

Topics

• Animals
• Antigen-Antibody Complex
• Avian Leukosis Virus (genetics)
• Cell Line
• Chickens
• Epitopes (analysis)
• Genes, Viral
• Immune Sera
• Oncogenes
• Viral Proteins (immunology)