Chlorogenic acid and coffee prevent hypoxia-induced retinal degeneration.

Abstract

This study explored whether chlorogenic acid (CGA) and coffee have protective effects against retinal degeneration. Under hypoxic conditions, the viability of transformed retinal ganglion (RGC-5) cells was significantly reduced by treatment with the nitric oxide (NO) donor S-nitroso-N-acetylpenicillamine (SNAP). However, pretreatment with CGA attenuated cell death in a concentration-dependent manner. In addition, CGA prevented the up-regulation of apoptotic proteins such as Bad and cleaved caspase-3. Similar beneficial effects of both CGA and coffee extracts were observed in mice that had undergone an optic nerve crush (ONC) procedure. CGA and coffee extract reduced cell death by preventing the down-regulation of Thy-1. Our in vitro and in vivo studies demonstrated that coffee and its major component, CGA, significantly reduce apoptosis of retinal cells induced by hypoxia and NO, and that coffee consumption may help in preventing retinal degeneration.

• Authors: Holim Jang, Hong Ryul Ahn, Hyoung Jo, Kyung-A Kim, Eun Ha Lee, Ki Won Lee, Sang Hoon Jung, Chang Y Lee
• Journal: Journal of agricultural and food chemistry (J Agric Food Chem) Vol. 62 Issue 1 Pg. 182-91 (Jan 08 2014) ISSN: 1520-5118 [Electronic] United States
• PMID: 24295042 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cannabinoids
• Coffee
• O 1057
• Plant Extracts
• Chlorogenic Acid

Topics

• Animals
• Apoptosis (drug effects)
• Cannabinoids (metabolism)
• Cell Survival (drug effects)
• Chlorogenic Acid (administration & dosage)
• Coffea (chemistry)
• Coffee (chemistry)
• Humans
• Hypoxia (complications)
• Male
• Mice
• Mice, Inbred ICR
• Plant Extracts (administration & dosage)
• Retinal Degeneration (etiology, metabolism, physiopathology, prevention & control)
• Retinal Ganglion Cells (cytology, drug effects, metabolism)