The correlation between morphology and the expression of TGF-β signaling pathway proteins and epithelial-mesenchymal transition-related proteins in synovial sarcomas.

Abstract

Synovial sarcoma (SS) is a malignant tumor of soft tissue and is noted for late local recurrence and metastasis. Aberrant epithelial-mesenchymal transition (EMT) has been implicated in the pathogenesis of diverse human malignancies. Immunohistochemical techniques were used to assess EMT-related proteins (E-cadherin, N-cadherin, β-catenin, Snail, and Slug) and the TGF-β1 pathway (TGF-β1 and Smad2/3) proteins expression in different histological subtypes and epithelial mesenchymal compositions of SS. The expression of cell-surface (E-cadherin) and cytoskeletal proteins (β-catenin) were higher significantly in biphasic SSs (BSSs) (70.4%, 51.9%) than MFSSs (both for 10%). Among monophasic fibrous SSs (MFSSs) samples, E-cadherin protein expression was negatively correlated with expression Snail, Slug, TGF-β1, and Smad2/3. The expression levels of Snail and Smad2/3 were correlated with the pTNM stage (I-II vs. III-IV; P=0.047, P=0.021) and TGF-β1 exhibited a tendency toward a positive correlation with pTNM stage (I-II vs. III-IV; P=0.052), but did not correlate with the histological grade (p>0.05). Interestingly, our data showed that expression of E-cadherin protein correlated with greater survival in SS patients. Overexpression of Snail, and TGF-β1 is associated with suppressed expression of E-cadherin in MFSSs, which supports the hypothesis that the MFSS subtype may have developed via neoplastic EMT.

• Authors: Yan Qi, Cui-Cui Wang, Yong-Lai He, Hong Zou, Chun-Xia Liu, Li-Juan Pang, Jian-Ming Hu, Jin-Fang Jiang, Wen-Jie Zhang, Feng Li
• Journal: International journal of clinical and experimental pathology (Int J Clin Exp Pathol) Vol. 6 Issue 12 Pg. 2787-99 ( 2013) ISSN: 1936-2625 [Electronic] United States
• PMID: 24294365 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, CD
• Biomarkers, Tumor
• CDH1 protein, human
• CDH2 protein, human
• CTNNB1 protein, human
• Cadherins
• SMAD2 protein, human
• SMAD3 protein, human
• SNAI1 protein, human
• Smad2 Protein
• Smad3 Protein
• Snail Family Transcription Factors
• TGFB1 protein, human
• Transcription Factors
• Transforming Growth Factor beta1
• beta Catenin

Topics

• Adolescent
• Adult
• Aged
• Antigens, CD (analysis)
• Biomarkers, Tumor (analysis)
• Cadherins (analysis)
• Child
• Epithelial-Mesenchymal Transition
• Female
• Humans
• Immunohistochemistry
• Kaplan-Meier Estimate
• Male
• Middle Aged
• Neoplasm Grading
• Neoplasm Staging
• Sarcoma, Synovial (chemistry, mortality, pathology)
• Signal Transduction
• Smad2 Protein (analysis)
• Smad3 Protein (analysis)
• Snail Family Transcription Factors
• Soft Tissue Neoplasms (chemistry, mortality, pathology)
• Transcription Factors (analysis)
• Transforming Growth Factor beta1 (analysis)
• Young Adult
• beta Catenin (analysis)