Expression of integrins α3β1 and α5β1 and GlcNAc β1,6 glycan branching influences metastatic melanoma cell migration on fibronectin.

Abstract

Acquisition of metastatic potential is accompanied by changes in cell surface N-glycosylation. One of the best-studied changes is increased expression of N-acetylglucosaminyltransferase V enzyme (GnT-V) and its products, β1,6-branched N-linked oligosaccharides, observed in the tumorigenesis of many cancers. In this study we demonstrate that during the transition from the vertical growth phase (VGP) (WM793 cell line) to the metastatic stage (WM1205Lu line), β1,6 glycosylation of melanoma cell surface proteins increases as a consequence of elevated expression of the GnT-V-encoding Mgat-5 gene. Treatment with swainsonine led to reduced cell motility on fibronectin in both cell lines; the effect was stronger in metastatic cells, probably due to the higher content of GlcNAc β1,6-branched glycans on the main fibronectin receptors - integrins α5β1 and α3β1. Our results show that GlcNAc β1,6 N-glycosylation of cell surface receptors, which increases with the aggressiveness of melanoma cells, is an important factor influencing melanoma cell migration.

• Authors: Ewa Pocheć, Marcelina Janik, Dorota Hoja-Łukowicz, Paweł Link-Lenczowski, Małgorzata Przybyło, Anna Lityńska
• Journal: European journal of cell biology (Eur J Cell Biol) Vol. 92 Issue 12 Pg. 355-62 (Dec 2013) ISSN: 1618-1298 [Electronic] Germany
• PMID: 24290991 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier GmbH. All rights reserved.

Chemical References

• Fibronectins
• Integrin alpha3beta1
• Integrin alpha5beta1
• Polysaccharides
• N-Acetylglucosaminyltransferases
• alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase
• Acetylglucosamine

Topics

• Acetylglucosamine (metabolism)
• Cell Movement
• Fibronectins (metabolism)
• Glycosylation
• Humans
• Integrin alpha3beta1 (genetics, metabolism)
• Integrin alpha5beta1 (genetics, metabolism)
• Melanoma (metabolism, pathology)
• N-Acetylglucosaminyltransferases (metabolism)
• Neoplasm Metastasis
• Polysaccharides (metabolism)