HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Impact of O6-methylguanine-DNA methyltransferase (MGMT) promoter methylation and MGMT expression on dacarbazine resistance of Hodgkin's lymphoma cells.

Abstract
We analyzed the methylation status of the O6-methylguanine-DNA methyltransferase (MGMT) promoter and mRNA expression in HL cells and assessed the response of these cells to dacarbazine. Expression of MGMT correlated with the presence of non-methylated promoters and cell lines with non-methylated promoters showed increased resistance against dacarbazine. KM-H2 cells expressed fusion transcripts between MGMT and proline-rich coiled-coil 2B (PRRC2B) but no wild type MGMT transcripts. Dacarbazine sensitivity suggested that fusion transcripts are translated into a protein with reduced functionality. MGMT promoter methylation predicts dacarbazine sensitivity of HL cells and it might be interesting to analyze this factor in HL patients.
AuthorsStefanie Kewitz, Martina Stiefel, Christof M Kramm, Martin S Staege
JournalLeukemia research (Leuk Res) Vol. 38 Issue 1 Pg. 138-43 (Jan 2014) ISSN: 1873-5835 [Electronic] England
PMID24284332 (Publication Type: Journal Article)
CopyrightCopyright © 2013 Elsevier Ltd. All rights reserved.
Chemical References
  • Antineoplastic Agents, Alkylating
  • Purines
  • Dacarbazine
  • O(6)-Methylguanine-DNA Methyltransferase
  • Azacitidine
  • lomeguatrib
Topics
  • Antineoplastic Agents, Alkylating (pharmacology)
  • Azacitidine (pharmacology)
  • Cell Line, Tumor
  • Cell Survival (drug effects, genetics)
  • DNA Methylation
  • Dacarbazine (antagonists & inhibitors, pharmacology)
  • Drug Resistance, Neoplasm (drug effects, genetics)
  • Drug Synergism
  • Gene Expression Regulation, Enzymologic (drug effects)
  • Gene Expression Regulation, Neoplastic (drug effects)
  • Hodgkin Disease (genetics, metabolism, pathology)
  • Humans
  • Immunoblotting
  • O(6)-Methylguanine-DNA Methyltransferase (genetics, metabolism)
  • Promoter Regions, Genetic (genetics)
  • Purines (pharmacology)
  • Reverse Transcriptase Polymerase Chain Reaction

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: