Apoptosis are induced in J774 macrophages upon phagocytosis and killing of Pseudomonas aeruginosa.

Abstract

Apoptosis induced by Pseudomonas aeruginosa in host cells plays a role in pathogenesis. However, little is known how the apoptosis of macrophages harboring P. aeruginosa affects the host or pathogen. In this study, the viability of J774 macrophages phagocytosing Pa IID1117 (elastase- and protease-positive) was significantly reduced (53.8±4.5%) 48 h after infection and cell death occurred via apoptosis as seen by Hoechst 33258 staining and terminal deoxynucleotidyl transferase-mediated d-UTP nick end labeling (TUNEL) assay. An activated subunit of caspase 3 was found in the cellular lysate. Lower colony counts in infected cells and effective intracellular killing of bacteria were accompanied by enhanced apoptosis. Caspase 3 inhibiter inhibited apoptosis but did not prevent cell death and the extracellular leakage of bacteria. The apoptosis of the macrophages that phagocytose P. aeruginosa therefore inhibits the intracellular growth and spread of this bacterium and is important in host defense against P. aeruginosa infections.

• Authors: Jianling Zhang, Ru Jiang, Wei Wang, Hisao Takayama, Yoshinori Tanaka
• Journal: Cellular immunology (Cell Immunol) 2013 Nov-Dec Vol. 286 Issue 1-2 Pg. 11-5 ISSN: 1090-2163 [Electronic] Netherlands
• PMID: 24270217 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier Inc. All rights reserved.

Chemical References

• Bacterial Proteins
• Cysteine Proteinase Inhibitors
• Oligopeptides
• benzoylcarbonyl-aspartyl-glutamyl-valyl-aspartyl-fluoromethyl ketone
• Peptide Hydrolases
• Pancreatic Elastase
• Caspase 3

Topics

• Animals
• Apoptosis (drug effects, immunology)
• Bacterial Proteins (genetics, metabolism)
• Caspase 3 (genetics, immunology)
• Cell Line
• Colony Count, Microbial
• Cysteine Proteinase Inhibitors (pharmacology)
• Gene Expression
• Host-Pathogen Interactions
• In Situ Nick-End Labeling
• Macrophages (drug effects, immunology, microbiology)
• Mice
• Oligopeptides (pharmacology)
• Pancreatic Elastase (genetics, metabolism)
• Peptide Hydrolases (genetics, metabolism)
• Phagocytosis (drug effects)
• Pseudomonas aeruginosa (physiology)