20-O-β-D-glucopyranosyl-20(S)-protopanaxadiol-fortified ginseng extract attenuates the development of atopic dermatitis-like symptoms in NC/Nga mice.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Ginseng and ginsenosides are frequently used in the treatment of chronic inflammatory diseases. Recently, 20-O-β-d-glucopyranosyl-20(S)-protopanaxadiol (GPD), the main metabolite of ginsenosides, was reported to have both anti-allergic and anti-pruritic effects. The immunomodulatory effects of GPD-fortified ginseng extract (GFGE) on atopic dermatitis (AD)-like symptoms in mice were investigated. This study was designed to investigate the preventive effect of GFGE on AD-like symptoms.

MATERIALS AND METHODS:

The effects of orally administered GFGE on Dermatophagoides farinae body extract (DFE)-induced AD-like symptoms in NC/Nga mice were assessed by analyzing dermatitis score, ear thickness, scratching time, skin histological changes, and serum level of macrophage-derived chemokine (MDC). In addition, splenocytes were isolated from the mice and stimulated with anti-CD3 and anti-CD28 monoclonal antibodies to produce cytokines.

RESULTS:

Oral administration of GFGE significantly attenuated DFE-induced increases in dermatitis score, ear thickness, scratching time, and severity of skin lesions in NC/Nga mice. GFGE treatment also reduced level of MDC in serum, infiltration of eosinophils and mast cells in skin, and production of cytokines in splenocytes.

CONCLUSIONS:

These results suggest that GFGE might ameliorate DFE-induced AD-like symptoms and be an alternative therapeutic agent for the prevention of AD.

• Authors: Jong Rhan Kim, Jinhwan Choi, Jiyoung Kim, Heejeung Kim, Heerim Kang, Eun Hye Kim, Jeong-Hwa Chang, Yeong-Eun Kim, Young Jin Choi, Ki Won Lee, Hyong Joo Lee
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 151 Issue 1 Pg. 365-71 ( 2014) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 24269244 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2013 Published by Elsevier Ireland Ltd.

Chemical References

• Anti-Allergic Agents
• Antigens, Dermatophagoides
• Cytokines
• Ginsenosides
• Immunosuppressive Agents
• Plant Extracts
• ginsenoside M1
• Tacrolimus

Topics

• Administration, Oral
• Animals
• Anti-Allergic Agents (chemistry, pharmacology)
• Antigens, Dermatophagoides (toxicity)
• Cytokines (metabolism)
• Dermatitis, Atopic (chemically induced, drug therapy)
• Female
• Ginsenosides (chemistry, pharmacology)
• Immunosuppressive Agents (pharmacology)
• Mice
• Mice, Inbred Strains
• Panax (chemistry)
• Plant Extracts (chemistry, pharmacology)
• Skin (drug effects, pathology)
• Spleen (cytology)
• Tacrolimus (pharmacology)