Cycline efficacy on the propagation of human prions in primary cultured neurons is strain-specific.

Abstract

In prion diseases, a major issue in therapeutic research is the variability of the effect between strains. Stimulated by the report of an antiprion effect in a scrapie model and by ongoing international clinical trials using doxycycline, we studied the efficacy of cyclines against the propagation of human prions. First, we successfully propagated various Creutzfeldt-Jakob disease (CJD) isolates (sporadic, variant, and iatrogenic CJD) in neuronal cultures expressing the human prion protein. Then, we found that doxycycline was the most effective compound, with important variations between isolates. Isolates from sporadic CJD, the most common form of prion disease, showed the highest sensitivity.

• Authors: Samia Hannaoui, Alexianne Gougerot, Nicolas Privat, Etienne Levavasseur, Nicolas Bizat, Jean-Jacques Hauw, Jean-Philippe Brandel, Stéphane Haïk
• Journal: The Journal of infectious diseases (J Infect Dis) Vol. 209 Issue 7 Pg. 1144-8 (Apr 01 2014) ISSN: 1537-6613 [Electronic] United States
• PMID: 24265435 (Publication Type: Journal Article)

Chemical References

• Prions
• Doxycycline

Topics

• Cells, Cultured
• Doxycycline (metabolism, pharmacology, therapeutic use)
• Humans
• Neurons (drug effects, metabolism)
• Prions (antagonists & inhibitors, drug effects)