Abstract |
Nutritional or dietary factors have drawn attention due to their potential as an effective chemopreventive agent, which is considered a more rational strategy in cancer treatment. This study was designed to evaluate the effect of IP₆ extracted from rice bran on azoxymethane- (AOM-) induced colorectal cancer (CRC) in rats. Initially, male Sprague Dawley rats were divided into 5 groups, with 6 rats in each group. The rats received two intraperitoneal (i.p.) injections of AOM in saline (15 mg/kg body weight) over a 2-week period to induce CRC. IP₆ was given in three concentrations, 0.2% (w/v), 0.5% (w/v), and 1.0% (w/v), via drinking water for 16 weeks. The deregulation of the Wnt/β- catenin signaling pathway and the expression of cyclooxygenase (COX)-2 have been implicated in colorectal tumorigenesis. β- Catenin and COX-2 expressions were analysed using the quantitative RT-PCR and Western blotting. Herein, we reported that the administration of IP₆ markedly suppressed the incidence of tumors when compared to the control. Interestingly, the administration of IP₆ had also markedly decreased β- catenin and COX-2 in colon tumors. Thus, the downregulation of β- catenin and COX-2 could play a role in inhibiting the CRC development induced by IP₆ and thereby act as a potent anticancer agent.
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Authors | Nurul Husna Shafie, Norhaizan Mohd Esa, Hairuszah Ithnin, Abdah Md Akim, Norazalina Saad, Ashok Kumar Pandurangan |
Journal | BioMed research international
(Biomed Res Int)
Vol. 2013
Pg. 681027
( 2013)
ISSN: 2314-6141 [Electronic] United States |
PMID | 24260743
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- Dietary Fiber
- Neoplasm Proteins
- beta Catenin
- Phytic Acid
- Cyclooxygenase 2
- Ptgs2 protein, rat
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Topics |
- Animals
- Antineoplastic Agents
(chemistry, isolation & purification, pharmacology)
- Colorectal Neoplasms
(chemically induced, metabolism, pathology, prevention & control)
- Cyclooxygenase 2
(metabolism)
- Dietary Fiber
- Male
- Neoplasm Proteins
(antagonists & inhibitors, metabolism)
- Neoplasms, Experimental
(chemically induced, metabolism, prevention & control)
- Oryza
(chemistry)
- Phytic Acid
(chemistry, isolation & purification, pharmacology)
- Rats
- Rats, Sprague-Dawley
- Wnt Signaling Pathway
(drug effects)
- beta Catenin
(metabolism)
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