Abstract | BACKGROUND: METHODS: Mice were randomized to receive an intra-tracheal instillation of a natural exogenous surfactant preparation (bLES, 50 mg/kg) or no treatment as a control. MV was then performed using the isolated and perfused mouse lung (IPML) set up. This model allowed for lung perfusion during MV. In experiment 1, mice were exposed to mechanical ventilation only (tidal volume =20 mL/kg, 2 hours). In experiment 2, hydrochloric acid or air was instilled intra-tracheally four hours before applying exogenous surfactant and ventilation (tidal volume =5 mL/kg, 2 hours). RESULTS: For both experiments, exogenous surfactant administration led to increased total and functional surfactant in the treated groups compared to the controls. Exogenous surfactant administration in mice exposed to MV only did not affect peak inspiratory pressure (PIP), lung IL-6 levels and the development of perfusate inflammation compared to non-treated controls. Acid injured mice exposed to conventional MV showed elevated PIP, lung IL-6 and protein levels and greater perfusate inflammation compared to air instilled controls. Instillation of exogenous surfactant did not influence the development of lung injury. Moreover, exogenous surfactant was not effective in reducing the concentration of inflammatory cytokines in the perfusate. CONCLUSIONS: The data indicates that exogenous surfactant did not mitigate ventilation-induced systemic inflammation in our models. Future studies will focus on altering surfactant composition to improve its immuno-modulating activity.
|
Authors | Valeria Puntorieri, Josh Qua Hiansen, Lynda A McCaig, Li-Juan Yao, Ruud A W Veldhuizen, James F Lewis |
Journal | BMC pulmonary medicine
(BMC Pulm Med)
Vol. 13
Pg. 67
(Nov 20 2013)
ISSN: 1471-2466 [Electronic] England |
PMID | 24256698
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Cytokines
- Eicosanoids
- Interleukin-6
- Pulmonary Surfactants
- Hydrochloric Acid
|
Topics |
- Acute Lung Injury
(chemically induced, metabolism, therapy)
- Animals
- Bronchoalveolar Lavage Fluid
(chemistry)
- Cytokines
(analysis, metabolism)
- Disease Models, Animal
- Eicosanoids
(analysis, metabolism)
- Hydrochloric Acid
- Inflammation
(etiology, metabolism, prevention & control)
- Interleukin-6
(analysis, metabolism)
- Lung
(metabolism, pathology)
- Male
- Mice
- Permeability
(drug effects)
- Positive-Pressure Respiration
(adverse effects)
- Pulmonary Surfactants
(therapeutic use)
- Total Lung Capacity
(drug effects)
|