VSL#3 probiotics provide protection against acute intestinal ischaemia/reperfusion injury.

Abstract

Acute intestinal ischaemia/reperfusion injury (AII/R) is an adaptive physiologic response during critical illness, involving mesenteric vasoconstriction and hypoperfusion. Prevention of AII/R in high risk patient populations would have a significant impact on morbidity and mortality. The purpose of this study was to investigate the protective effects of VSL#3 probiotic treatment in a murine model of AII/R. Adult 129/SvEv mice were subjected to an experimental AII/R model using superior mesenteric artery occlusion. Animals were pre-treated with either three days or two weeks of VSL#3 probiotics. Local tissue injury markers were assessed by levels of myeloperoxidase and activation of nuclear factor kappa B (NFкB). Systemic and local cytokines, including interleukin (IL)-1β, IL- 10, TNFα, and interferon gamma were measured by ELISA and multiplex fluorescent detection. VSL#3 probiotics reduced local tissue inflammation and injury due to AII/R. A two-week course of VSL#3 was more effective than a shorter three-day course. The reduction in local inflammation from the two-week course of VSL#3 is correlated to a significant reduction in levels of active IL-1β, and tissue levels of myeloperoxidase. Levels of active NFкB were significantly elevated in the vehicle-fed AII/R mice, corroborating with tissue inflammation, which were attenuated by VSL#3 administrations. VSL#3 did not cause any systemic inflammation or lung injury. VSL#3 probiotics are effective in reducing local tissue injury from AII/R by down-regulating pro-inflammatory mediators and immune cell recruitment. This study highlights a potential role for VSL#3 in management of patients at high risk for AII/R.

• Authors: S Y Salim, P Y Young, C M Lukowski, K L Madsen, B Sis, T A Churchill, R G Khadaroo
• Journal: Beneficial microbes (Benef Microbes) Vol. 4 Issue 4 Pg. 357-65 (Dec 01 2013) ISSN: 1876-2891 [Electronic] Netherlands
• PMID: 24240573 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cytokines
• NF-kappa B
• Peroxidase

Topics

• Animals
• Cytokines (analysis)
• Disease Models, Animal
• Intestinal Diseases (prevention & control)
• Ischemia (complications)
• Mice
• NF-kappa B (analysis)
• Peroxidase (analysis)
• Probiotics (therapeutic use)
• Reperfusion Injury (pathology, prevention & control)
• Treatment Outcome