HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Direct detection of hyaluronidase in urine using cationic gold nanoparticles: a potential diagnostic test for bladder cancer.

Abstract
Hyaluronidase (HAase) was reported as a urinary marker of bladder cancer. In this study, a simple colorimetric gold nanoparticle (AuNP) assay was developed for rapid and sensitive detection of urinary HAase activity. Charge interaction between polyanionic hyaluronic acid (HA) and cationic AuNPs stabilized with cetyl trimethyl ammonium bromide (CTAB) led to formation of gold aggregates and a red to blue color shift. HAase digests HA into small fragments preventing the aggregation of cationic AuNPs. The nonspecific aggregation of AuNPs in urine samples was overcome by pre-treatment of samples with the polycationic chitosan that was able to agglomerate all negatively charged interfering moieties before performing the assay. The developed AuNP assay was compared with zymography for qualitative detection of urinary HAase activity in 40 bladder carcinoma patients, 11 benign bladder lesions patients and 15 normal individuals, the assay sensitivity was 82.5% vs. 65% for zymography, while the specificity for both assays was 96.1%. The absorption ratio, A530/A620 of the reacted AuNP solution was used to quantify the HAase activity. The best cut off value was 93.5 μU/ng protein, at which the sensitivity was 90% and the specificity was 80.8%.The developed colorimetric AuNP HAase assay is simple, inexpensive, and can aid noninvasive diagnosis of bladder cancer.
AuthorsAhmed Ibrahim Nossier, Sanaa Eissa, Manal Fouad Ismail, Mohamed Ahmed Hamdy, Hassan Mohamed El-Said Azzazy
JournalBiosensors & bioelectronics (Biosens Bioelectron) Vol. 54 Pg. 7-14 (Apr 15 2014) ISSN: 1873-4235 [Electronic] England
PMID24240162 (Publication Type: Evaluation Study, Journal Article)
Copyright© 2013 Elsevier B.V. All rights reserved.
Chemical References
  • Biomarkers, Tumor
  • Gold
  • Hyaluronoglucosaminidase
Topics
  • Biomarkers, Tumor (urine)
  • Colorimetry (economics, methods)
  • Gold (chemistry)
  • Humans
  • Hyaluronoglucosaminidase (urine)
  • Nanoparticles (chemistry)
  • Sensitivity and Specificity
  • Urinary Bladder Neoplasms (diagnosis, urine)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: