Modifications of mesenteric adipose tissue during moderate experimental colitis in mice.

Abstract

AIMS:

Adipose tissue secretes various proteins referred to as adipokines, being involved in inflammation. It was recognized that mesenteric adipose tissue (MAT) is altered by inflammation, and pathologies such as inflammatory bowel disease (IBD). The aim of this study was to investigate the alterations of the mesenteric adipose tissue in two experimental colitis models in mice adapted to obtain moderate colonic inflammation.

MAIN METHODS:

Colonic inflammation was obtained using two models, either DSS dissolved in drinking water or intra-colonic instillation of DNBS. The expression of adipokines (leptin and adiponectin) and inflammatory markers (IL-6, MCP-1, F4/80) was studied by qRT-PCR in the MAT of treated and control mice.

KEY FINDINGS:

Observations of the colon and IL-6 plasma level determination demonstrated that DNBS treatment led to stronger inflammation. Colitis induced a decrease of mRNA encoding to leptin and adiponectin in MAT. In contrast, colonic inflammation led to an increase of mRNA encoding to IL-6, MCP-1 and F4/80, a specific marker of macrophages.

SIGNIFICANCE:

The mesenteric adipose tissue, in two models of moderate colitis, shows a loss of adipose profile and a strong increase of inflammatory pattern, close to the observations made in MAT of IBD patients. These data suggest that these pro-inflammatory modifications of MAT have to be taken into account in the pathophysiology of IBD.

• Authors: Isabelle Olivier, Vassilia Theodorou, Philippe Valet, Isabelle Castan-Laurell, Laurent Ferrier, Hélène Eutamène
• Journal: Life sciences (Life Sci) Vol. 94 Issue 1 Pg. 1-7 (Jan 14 2014) ISSN: 1879-0631 [Electronic] Netherlands
• PMID: 24215755 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Inc. All rights reserved.

Chemical References

• Adiponectin
• Inflammation Mediators
• Leptin
• RNA, Messenger
• 2,4-dinitrofluorobenzene sulfonic acid
• Dextran Sulfate
• Dinitrofluorobenzene
• Proteasome Endopeptidase Complex

Topics

• Adiponectin (metabolism)
• Adipose Tissue (metabolism)
• Animals
• Colitis (physiopathology)
• Dextran Sulfate (toxicity)
• Dinitrofluorobenzene (analogs & derivatives, toxicity)
• Disease Models, Animal
• Inflammation (physiopathology)
• Inflammation Mediators (metabolism)
• Inflammatory Bowel Diseases (physiopathology)
• Leptin (metabolism)
• Male
• Mice
• Mice, Inbred BALB C
• Proteasome Endopeptidase Complex (metabolism)
• RNA, Messenger (metabolism)
• Reverse Transcriptase Polymerase Chain Reaction