Abstract | CONTEXT: The mechanisms responsible for impaired chylomicron metabolism have not been adequately investigated in obese subjects. OBJECTIVE: We aimed to compare apolipoprotein ( apo) B-48 kinetics in obese and lean men by developing a new model to describe the kinetics of apoB-48 particles in the postprandial state. DESIGN, SETTING, AND PATIENTS: Seven obese and 13 age-matched lean men were given an oral fat load. apoB-48 tracer to tracee ratios were measured after intravenous d3-leucine administration using gas chromatography-mass spectrometry. Kinetic parameters were derived using a multicompartmental model. OUTCOMES MEASURES: Plasma total and incremental apoB-48 0-10 hour areas under the curve as well as apoB-48 secretion and fractional catabolic rate. RESULTS: Compared with lean men, fasting plasma triglyceride (+148%) and apoB-48 (+110%) concentrations as well as plasma total and incremental triglycerides (+184% and +185%, respectively) and apoB-48 (+182% and 224%, respectively) areas under the curve were significantly higher in obese men (P<.05 for all). The obese men also had significantly (P<.05 for all) higher secretion rates of apoB-48 in the fasted state (+145%) as well as at 3 hours (+70%), 4 hours (+82%), 5 hours (+82%), 6 hours (+76%), and 8 hours (+61%) in response to the fat load. This was associated with a greater number of apoB-48-containing particles secreted over the 10-hour study period in the obese men, compared with lean men (+125%, P<.01). The fractional catabolic rate of apoB-48 was significantly lower in the obese men compared with the lean men (-33%, P<.05) CONCLUSION: We demonstrate that postprandial hypertriglyceridemia in central obesity relates to an overproduction and impaired catabolism of apoB-48-containing lipoproteins. These findings are based on a new, physiologically relevant, kinetic model, which describes the non-steady-state postprandial metabolism of apoB-48.
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Authors | Annette T Y Wong, Dick C Chan, Jing Pang, Gerald F Watts, P Hugh R Barrett |
Journal | The Journal of clinical endocrinology and metabolism
(J Clin Endocrinol Metab)
Vol. 99
Issue 1
Pg. E122-6
(Jan 2014)
ISSN: 1945-7197 [Electronic] United States |
PMID | 24203058
(Publication Type: Controlled Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Apolipoprotein B-48
- Blood Proteins
- Radioactive Tracers
- Leucine
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Topics |
- Aged
- Apolipoprotein B-48
(metabolism)
- Blood Proteins
(metabolism)
- Humans
- Isotope Labeling
(methods)
- Kinetics
- Leucine
(pharmacokinetics)
- Male
- Middle Aged
- Obesity
(metabolism)
- Postprandial Period
(physiology)
- Protein Transport
- Radioactive Tracers
- Thinness
(metabolism)
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