Analysis of reference gene stability after Israeli acute paralysis virus infection in bumblebees Bombus terrestris.

Abstract

To date, there are no validated internal reference genes for the normalization of RT-qPCR data from virus infection experiments with pollinating insects. In this study we evaluated the stability of five candidate internal reference genes: elongation factor-1-alpha (ELF1α), peptidylprolyl isomerase A (PPIA), 60S ribosomal protein L23 (RPL23), TATA-binding protein (TBP) and polyubiquitin (UBI), in relation to Israeli acute paralysis virus (IAPV) infection of Bombus terrestris. We investigated the stability of these genes: in whole bodies and individual body parts, as well as in whole bodies collected at different time intervals after infection with IAPV. Our data identified PPIA as the single, most-optimal internal reference gene and the combination of PPAI-RPL23-UBI as a fully-sufficient multiple internal reference genes set for IAPV infection experiments in B. terrestris.

• Authors: Jinzhi Niu, Kaat Cappelle, Joachim R de Miranda, Guy Smagghe, Ivan Meeus
• Journal: Journal of invertebrate pathology (J Invertebr Pathol) Vol. 115 Pg. 76-9 (Jan 2014) ISSN: 1096-0805 [Electronic] United States
• PMID: 24184950 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier Inc. All rights reserved.

Topics

• Animals
• Bees (genetics, virology)
• Dicistroviridae
• Genes, Insect (genetics)
• Real-Time Polymerase Chain Reaction
• Reference Standards
• Reverse Transcriptase Polymerase Chain Reaction