HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Performance of anti-HEV assays for diagnosing acute hepatitis E in immunocompromised patients.

Abstract
Hepatitis E virus is an emerging concern in immunocompromised patients, who may become chronically infected. This prompted us to assess the performance of two anti-HEV IgG and IgM assays for diagnosing acute HEV infections. The specificities of the assays were estimated by testing samples from 2 to 3 year-old French children and blood donors and their sensitivities by testing 40 immunocompromised patients acutely infected. Both anti-HEV IgM assays were highly specific (99.6% and 100%). The sensitivity of the Adaltis was 87.5%, and that of Wantai was 85%. The specificities of anti-HEV IgG Wantai (97.8%) and Adaltis tests (89.5%, p=0.1) were similar but the Wantai test was more sensitive (45%) than the Adaltis test (15%, p<0.001). None of the samples was anti-HEV IgM negative and IgG positive. We conclude that these anti-HEV IgM assays performed well in immunosuppressed subjects with acute hepatitis E and can be used as first line virological tools. Testing for anti-HEV IgG and IgM simultaneously at the acute phase did not improve the diagnostic performance. In contrast, molecular detection of HEV RNA appears essential to exclude an HEV infection in patients who are negative for anti-HEV IgM and to assess the evolution of hepatitis E 3 months thereafter.
AuthorsFlorence Abravanel, Sabine Chapuy-Regaud, Sébastien Lhomme, Marcel Miedougé, Jean-Marie Peron, Laurent Alric, Lionel Rostaing, Nassim Kamar, Jacques Izopet
JournalJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology (J Clin Virol) Vol. 58 Issue 4 Pg. 624-8 (Dec 2013) ISSN: 1873-5967 [Electronic] Netherlands
PMID24183927 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2013 Elsevier B.V. All rights reserved.
Chemical References
  • Hepatitis Antibodies
  • Immunoglobulin G
  • Immunoglobulin M
Topics
  • Hepatitis Antibodies (blood)
  • Hepatitis E (diagnosis, immunology)
  • Humans
  • Immunocompromised Host (immunology)
  • Immunoenzyme Techniques (standards)
  • Immunoglobulin G (blood)
  • Immunoglobulin M (blood)
  • Sensitivity and Specificity
  • Serologic Tests (standards)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: