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Biochemical and immunological characterization of Suncus murinus mammary tumor virus DNA polymerase.

Abstract
Enzymological properties of the RNA-directed DNA polymerase associated with the Suncus murinus mammary tumor virus (Sm-MTV) was investigated and its antigenic relatedness to other retroviral DNA polymerases was examined. The enzyme exhibited higher activity in the presence of Mg2+ than in the presence of Mn2+ with endogenous RNA as well as with almost all of the synthetic template X primers tested. Mg2+ was also effective with poly(2'-O-methylcytidylate) X oligodeoxyguanylate which was known to be specific for Mn2+. To examine the immunological relatedness of this enzyme with other retroviral DNA polymerases, remaining Sm-MTV DNA polymerase activity was measured after treatment of this enzyme with various antisera prepared against each of the reverse transcriptases of Mason-Pfizer monkey virus (MPMV), murine mammary tumor virus (MuMTV), simian sarcoma virus-simian sarcoma associated virus (SSV/SSAV), and Rauscher murine leukemia virus (RLV). No inhibition of the Sm-MTV enzyme activity was observed when treated with the latter three antisera with which the DNA polymerase activities of the corresponding retroviruses were fully inhibited. Only the antiserum against MPMV-enzyme, however, was found to slightly inhibit the Sm-MTV enzyme activity. These results indicate that Sm-MTV DNA polymerase has similar enzymological properties to those of MPMV and MuMTV and shares some common antigenic determinant group(s) with MPMV DNA polymerase.
AuthorsT Yokoi, H Nakane, S Hayakawa, S Saga, S Nagayoshi, Y Tsutsui, A Aoyama, M Hoshino, K Ono
JournalVirology (Virology) Vol. 148 Issue 2 Pg. 268-74 (Jan 30 1986) ISSN: 0042-6822 [Print] United States
PMID2417416 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Viral Proteins
  • Manganese
  • RNA-Directed DNA Polymerase
  • Magnesium
Topics
  • Animals
  • Cell Line
  • Magnesium (metabolism)
  • Mammary Glands, Animal
  • Manganese (metabolism)
  • Neoplasms (microbiology, veterinary)
  • RNA-Directed DNA Polymerase (immunology, isolation & purification, metabolism)
  • Retroviridae (classification, enzymology, isolation & purification)
  • Shrews (microbiology)
  • Substrate Specificity
  • Viral Proteins (immunology, isolation & purification, metabolism)

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