Abstract |
Enzymological properties of the RNA-directed DNA polymerase associated with the Suncus murinus mammary tumor virus (Sm-MTV) was investigated and its antigenic relatedness to other retroviral DNA polymerases was examined. The enzyme exhibited higher activity in the presence of Mg2+ than in the presence of Mn2+ with endogenous RNA as well as with almost all of the synthetic template X primers tested. Mg2+ was also effective with poly(2'-O-methylcytidylate) X oligodeoxyguanylate which was known to be specific for Mn2+. To examine the immunological relatedness of this enzyme with other retroviral DNA polymerases, remaining Sm-MTV DNA polymerase activity was measured after treatment of this enzyme with various antisera prepared against each of the reverse transcriptases of Mason-Pfizer monkey virus (MPMV), murine mammary tumor virus (MuMTV), simian sarcoma virus-simian sarcoma associated virus (SSV/SSAV), and Rauscher murine leukemia virus (RLV). No inhibition of the Sm-MTV enzyme activity was observed when treated with the latter three antisera with which the DNA polymerase activities of the corresponding retroviruses were fully inhibited. Only the antiserum against MPMV- enzyme, however, was found to slightly inhibit the Sm-MTV enzyme activity. These results indicate that Sm-MTV DNA polymerase has similar enzymological properties to those of MPMV and MuMTV and shares some common antigenic determinant group(s) with MPMV DNA polymerase.
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Authors | T Yokoi, H Nakane, S Hayakawa, S Saga, S Nagayoshi, Y Tsutsui, A Aoyama, M Hoshino, K Ono |
Journal | Virology
(Virology)
Vol. 148
Issue 2
Pg. 268-74
(Jan 30 1986)
ISSN: 0042-6822 [Print] United States |
PMID | 2417416
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Viral Proteins
- Manganese
- RNA-Directed DNA Polymerase
- Magnesium
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Topics |
- Animals
- Cell Line
- Magnesium
(metabolism)
- Mammary Glands, Animal
- Manganese
(metabolism)
- Neoplasms
(microbiology, veterinary)
- RNA-Directed DNA Polymerase
(immunology, isolation & purification, metabolism)
- Retroviridae
(classification, enzymology, isolation & purification)
- Shrews
(microbiology)
- Substrate Specificity
- Viral Proteins
(immunology, isolation & purification, metabolism)
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