Considerable evidence shows a key role for
protein modification in the adverse effects of chemicals; however, the interaction of
diesel exhaust particles (
DEP) with
proteins and the resulting
biological activity remains unclear.
DEP and
carbon black (CB)
suspensions with and without
bovine serum albumin (BSA) were used to elucidate the
biological effects of
air pollutants. The
DEP and CB samples were then divided into
suspensions and supernatants. Two important goals of the interaction of
DEP with BSA were as follows: (1) understanding BSA modification by particles and (2) investigating the effects of particles bound with BSA and the corresponding supernatants on cellular oxidative stress and
inflammation. We observed significant free amino groups production was caused by
DEP. Using liquid chromatography-mass spectrometry (LC-MS), we observed that BSA was significantly oxidised by
DEP in the supernatants and that the
peptides ETYGDMADCCEK, MPCTEDYLSLILNR and TVMENFVAFVDK, derived BSA-
DEP conjugates, were also oxidised. In A549 cells,
DEP-BSA
suspensions and the corresponding supernatants reduced
8-hydroxy-2'-deoxyguanosine (8-OHdG) production and increased
interleukin-6 (IL-6) levels when compared to
DEP solutions without BSA. Our findings suggest that oxidatively modified forms of BSA caused by
DEP could lead to oxidative stress and the activation of
inflammation.