Experiment research on inhibition of glioma with sTRAIL in vitro.

Abstract

We report that adenovirus mediated TNF-related apoptosis-inducing ligand (TRAIL) influenced the cell growth and cell cycle in the glioma cells in vitro. After being infected with the Ad-sTRAIL, U251 cell growth was inhibited. The expression of sTRAIL was detected using immunofluorescence. The higher rate of apoptosis was demonstrated using short-term microculture tetrazoliun (MTT) assay and flow cytometry. The rate of Ad-sTRAIL-inducing U251 cell apoptosis was increased depending on the dosage and the time. The apoptosis of G0/G1 and S phase cells was more significant than that of the control groups. The growth and proliferation of U251 cell line was inhibited after the infection of Ad-sTRAIL. It is dose- and time dependent.

• Authors: Yihe Dou, Yangang Wang, Jian Xu, Zhaojian Li, Peng Sun, Qinghai Meng
• Journal: Artificial cells, nanomedicine, and biotechnology (Artif Cells Nanomed Biotechnol) Vol. 42 Issue 3 Pg. 186-91 (Jun 2014) ISSN: 2169-141X [Electronic] England
• PMID: 24156316 (Publication Type: Journal Article)

Chemical References

• TNF-Related Apoptosis-Inducing Ligand
• DNA

Topics

• Adenoviridae (genetics)
• Apoptosis
• Cell Cycle
• Cell Line, Tumor
• Cell Proliferation
• Cell Survival
• DNA (metabolism)
• Flow Cytometry
• Genetic Vectors (genetics)
• Glioma (genetics, metabolism, pathology)
• Humans
• TNF-Related Apoptosis-Inducing Ligand (genetics, metabolism)
• Time Factors