Altered expression of miR-24, miR-126 and miR-365 does not affect viability of childhood TCF3-rearranged leukemia cells.

Abstract

Among the microRNAs (miRNAs) that control different cellular processes, miR-24, miR-126 and miR-365 were shown to regulate cell cycle progression and apoptosis in various types of tumors. Interestingly, these three miRNAs were downregulated in pediatric TCF3-rearranged B-cell precursor acute lymphoblastic leukemia (BCP-ALL). Here, we showed that individual or combined overexpression of miR-24, miR-126 and miR-365 can neither alter the cell cycle progression nor the amount of apoptosis in 697, KASUMI-2 or MHH-CALL-3 TCF3-rearranged leukemic cells. We further integrated the miRNA-mRNA expression data of 37 children with BCP-ALL to identify candidate target genes for these three miRNAs. However, the expression levels of selected candidate target genes (ELL, EBF3 and IRF4 for miR-24, PITPNC1 for miR-126 and ZAP-70 for miR-365) did not reduce upon miRNAs overexpression in MHH-CALL-3 TCF3-rearranged leukemic cells. Although the expression level of AURKB-a validated target for miR-24-was reduced upon miR-24 overexpression in hepatocarcinoma HEP-G2 cells, overexpression of miR-24 cannot alter AURKB expression levels in MHH-CALL-3 TCF3-rearranged leukemic cells. Taken together, our data suggest that miRNAs' function is highly tissue-dependent and that a defined biological target gene or function of one miRNA in a specific tissue cannot be extended as a generalized target/function for that miRNA in all types of cells/tissues.

• Authors: F Akbari Moqadam, J M Boer, E A M Lange-Turenhout, R Pieters, M L den Boer
• Journal: Leukemia (Leukemia) Vol. 28 Issue 5 Pg. 1008-14 (May 2014) ISSN: 1476-5551 [Electronic] England
• PMID: 24153013 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Validation Study)

Chemical References

• Basic Helix-Loop-Helix Transcription Factors
• DNA Primers
• MicroRNAs
• TCF3 protein, human

Topics

• Apoptosis
• Base Sequence
• Basic Helix-Loop-Helix Transcription Factors (genetics)
• Cell Cycle (genetics)
• Cell Line, Tumor
• DNA Primers
• Gene Rearrangement
• Humans
• MicroRNAs (genetics)
• Precursor Cell Lymphoblastic Leukemia-Lymphoma (genetics, pathology)
• Real-Time Polymerase Chain Reaction