Treatment of Brucella canis and Brucella abortus in vitro and in vivo by stable plurilamellar vesicle-encapsulated aminoglycosides.

Abstract	Stable plurilamellar vesicles (SPLVs) entrapping aminoglycosides were used to treat infections due to Brucella species (Brucella canis and Brucella abortus). SPLV-entrapped antibiotics effectively eliminated internalized B. canis in cultures of resident murine peritoneal macrophages and internalized B. abortus in cultures of resident guinea pig peritoneal macrophages. In vivo studies demonstrated that SPLV-entrapped aminoglycosides administered to B. canis-infected mice and B. abortus-infected guinea pigs effectively eliminated bacteria from infected organs. The dosage schedule used involved two intraperitoneal administrations of SPLV-entrapped aminoglycosides at three-day intervals. The results demonstrate the superiority of SPLV-entrapped aminoglycosides to free aminoglycosides in effecting elimination of facultative intracellular bacteria in vitro and in vivo. The use of SPLVs as a drug carrier has broad application to treatment of infections due to other organisms.
Authors	M W Fountain, S J Weiss, A G Fountain, A Shen, R P Lenk
Journal	The Journal of infectious diseases (J Infect Dis) Vol. 152 Issue 3 Pg. 529-35 (Sep 1985) ISSN: 0022-1899 [Print] United States
PMID	2411828 (Publication Type: Journal Article)
Chemical References	Aminoglycosides Anti-Bacterial Agents Gentamicins Liposomes Kanamycin Dihydrostreptomycin Sulfate Streptomycin
Topics	Aminoglycosides (pharmacology, therapeutic use) Animals Anti-Bacterial Agents (pharmacology, therapeutic use) Brucella (drug effects) Brucella abortus (drug effects) Brucellosis (drug therapy, microbiology) Cells, Cultured Dihydrostreptomycin Sulfate (pharmacology, therapeutic use) Female Gentamicins (pharmacology, therapeutic use) Guinea Pigs Kanamycin (pharmacology, therapeutic use) Liposomes (administration & dosage) Macrophages (microbiology) Male Mice Mice, Inbred C57BL Spleen (microbiology) Streptomycin (pharmacology, therapeutic use)

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