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Anti-inflammatory R-prostaglandins from Caribbean Colombian soft coral Plexaura homomalla.

AbstractOBJECTIVES:
This study aims to evaluate the effect of prostaglandins isolated from soft coral Plexaura homomalla, collected in Colombian Caribbean Sea, on in vivo and in vitro inflammation models.
METHODS:
Extracts from P. homomalla were fractionated and sequentially chromatographed to obtain the prostaglandins: (15R)-PGA2 (1), (15R)-PGA2 -Me (2), (15R)-O-Ac-PGA2 (3), (15R)-O-Ac-PGA2 -Me (4) and (15R)-PGE2 (5) in addition to three semi-synthetic prostaglandins obtained by transformations of the natural products. The anti-inflammatory properties of natural and semi-synthetic compounds were determined in vivo using 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mouse ear oedema model and in vitro leucocyte degranulation, myeloperoxidase (MPO) and elastase enzymatic activities from human polymorphonuclear cells (PMNs). The cell viability was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.
KEY FINDINGS:
In the in vivo assay, (15R)-PGE2 (1) and (15R)-O-Ac-PGA2 (3) showed anti-inflammatory activity, as well as in vitro inhibition of elastase release from PMNs. In the PMNs degranulation assay, (15R)-PGE2 (5), was the most active compound in the inhibition of MPO release. Finally, all the tested prostaglandins showed moderate inhibition for elastase enzyme activity, whereas none of the prostaglandins exhibit significative inhibition on MPO activity.
CONCLUSION:
(15R)-PGE2 (1) and (15R)-O-Ac-PGA2 (3) present significant inhibition on three important events related to the topical inflammatory response induced by TPA: the oedema formation, the PMNs degranulation, events that modulate MPO and elastase levels at inflammation site, and the inhibition of the enzyme activity.
AuthorsEduardo Reina, Freddy A Ramos, Leonardo Castellanos, Marcela Aragón, Luis F Ospina
JournalThe Journal of pharmacy and pharmacology (J Pharm Pharmacol) Vol. 65 Issue 11 Pg. 1643-52 (Nov 2013) ISSN: 2042-7158 [Electronic] England
PMID24118067 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Copyright© 2013 Royal Pharmaceutical Society.
Chemical References
  • Anti-Inflammatory Agents
  • Biological Products
  • Enzyme Inhibitors
  • Prostaglandins
  • Peroxidase
  • Pancreatic Elastase
  • Tetradecanoylphorbol Acetate
Topics
  • Animals
  • Anthozoa (chemistry)
  • Anti-Inflammatory Agents (isolation & purification, pharmacology, therapeutic use)
  • Biological Products (chemistry, pharmacology, therapeutic use)
  • Caribbean Region
  • Cell Degranulation (drug effects)
  • Colombia
  • Edema (chemically induced, drug therapy)
  • Enzyme Inhibitors (pharmacology, therapeutic use)
  • Humans
  • Inflammation (chemically induced, drug therapy, metabolism)
  • Mice
  • Mice, Inbred ICR
  • Neutrophil Infiltration (drug effects)
  • Neutrophils (metabolism)
  • Pancreatic Elastase (metabolism)
  • Peroxidase (metabolism)
  • Prostaglandins (isolation & purification, pharmacology, therapeutic use)
  • Tetradecanoylphorbol Acetate

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