Overexpression of insulin-like growth factor-I receptor as a pertinent biomarker for hepatocytes malignant transformation.
Abstract | AIM: METHODS:
Hepatoma models were made by inducing with 2-fluorenylacetamide (2-FAA) on male Sprague-Dawley rats. Morphological changes of hepatocytes were observed by pathological Hematoxylin and eosin staining, the dynamic expressions of liver and serum IGF-IR were quantitatively analyzed by an enzyme-linked immunosorbent assay. The distribution of hepatic IGF-IR was located by immunohistochemistry. The fragments of IGF-IR gene were amplified by reverse transcription-polymerase chain reaction, and confirmed by sequencing. RESULTS: Rat hepatocytes after induced by 2-FAA were changed dynamically from granule-like degeneration, precancerous to hepatoma formation with the progressing increasing of hepatic mRNA or IGF-IR expression. The incidences of liver IGF-IR, IGF-IR mRNA, specific IGF-IR concentration (ng/mg wet liver), and serum IGF-IR level (ng/mL) were 0.0%, 0.0%, 0.63 ± 0.17, and 1.33 ± 0.47 in the control; 50.0%, 61.1%, 0.65 ± 0.2, and 1.51 ± 0.46 in the degeneration; 88.9%, 100%, 0.66 ± 0.14, and 1.92 ± 0.29 in the precancerosis; and 100%, 100%, 0.96 ± 0.09, and 2.43 ± 0.57 in the cancerous group, respectively. IGF-IR expression in the cancerous group was significantly higher (P < 0.01) than that in any of other groups at mRNA or protein level. The closely positive IGF-IR relationship was found between livers and sera (r = 0.91, t = 14.222, P < 0.01), respectively. CONCLUSION: IGF-IR expression may participate in rat hepatocarcinogenesis and its abnormality should be an early marker for hepatocytes malignant transformation.
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Authors | Xiao-Di Yan, Min Yao, Li Wang, Hai-Jian Zhang, Mei-Juan Yan, Xing Gu, Yun Shi, Jie Chen, Zhi-Zhen Dong, Deng-Fu Yao |
Journal | World journal of gastroenterology
(World J Gastroenterol)
Vol. 19
Issue 36
Pg. 6084-92
(Sep 28 2013)
ISSN: 2219-2840 [Electronic] United States |
PMID | 24106410
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers, Tumor
- RNA, Messenger
- 2-Acetylaminofluorene
- Receptor, IGF Type 1
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Topics |
- 2-Acetylaminofluorene
- Animals
- Biomarkers, Tumor
(blood, genetics, metabolism)
- Carcinoma, Hepatocellular
(chemically induced, genetics, metabolism, pathology)
- Cell Transformation, Neoplastic
(chemically induced, genetics, metabolism, pathology)
- Disease Models, Animal
- Gene Expression Regulation, Neoplastic
- Hepatocytes
(metabolism, pathology)
- Liver Neoplasms
(chemically induced, genetics, metabolism, pathology)
- Male
- RNA, Messenger
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Receptor, IGF Type 1
(blood, genetics, metabolism)
- Up-Regulation
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