Eosinophil depletion protects mice from tongue squamous cell carcinoma induced by 4-nitroquinoline-1-oxide.

Abstract

AIMS:

Tumor-associated tissue eosinophilia (TATE) has been correlated with prognosis in oral squamous cell carcinoma (OSCC). This study aimed to investigate whether eosinophils depletion affects experimental oral carcinogenesis.

METHODS AND RESULTS:

BALB/c (wild type - WT) and eosinophil-deficient (Δdb/GATA-1) mice were treated with the carcinogen 4-nitroquinoline-1-oxide (4NQO) in drinking water for 28 weeks. Tongues were collected for histopathological and immunohistochemical analysis, as well as for the evaluation of cytokines/chemokines by ELISA. The tongue SCC induced by 4NQO was associated with a rise in eosinophil numbers. WT-treated group showed a significantly increased incidence of SCC, with higher cytological atypia, in comparison with Δdb/GATA-1 mice. Consistently, the proliferative index was higher in WT compared to the Δdb/GATA-1-treated group. No significant changes in the concentration of CCL3, CCL11 and TNF-α were detected for both groups after 4NQO treatment.

CONCLUSIONS:

These results suggest that eosinophils might be responsible for the deleterious outcome of experimental tongue carcinogenesis, given that their ablation protects mice from OSCC.

• Authors: Janine Mayra da Silva, Celso Martins Queiroz-Junior, Aline Carvalho Batista, Milene Alvarenga Rachid, Mauro Martins Teixeira, Tarcília Aparecida da Silva
• Journal: Histology and histopathology (Histol Histopathol) Vol. 29 Issue 3 Pg. 387-96 (Mar 2014) ISSN: 1699-5848 [Electronic] Spain
• PMID: 24105297 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Carcinogens
• 4-Nitroquinoline-1-oxide

Topics

• 4-Nitroquinoline-1-oxide (toxicity)
• Animals
• Carcinogens (toxicity)
• Carcinoma, Squamous Cell (chemically induced, pathology)
• Disease Models, Animal
• Enzyme-Linked Immunosorbent Assay
• Eosinophils (pathology)
• Immunohistochemistry
• Mice
• Mice, Inbred BALB C
• Mice, Mutant Strains
• Tongue Neoplasms (chemically induced, pathology)