Effects of vinorelbine on cisplatin resistance reversal in human lung cancer A549/DDP cells.

Abstract

Multi-drug resistance (MDR) is an essential aspect of human lung cancer chemotherapy failure. Recent studies have shown that vinorelbine is involved in underlying processes in human tumors, reversing the MDR inseveral types of cancer cells. However, the roles and potential mechanism are not fully clear. In this study, we explored effects of vinorelbine in multi-drug resistance reversal of human lung cancer A549/DDP cells. We found that vinorelbine increased drug sensitivity to cisplatin and intracellular accumulation of rhodamine-123, while decreasing expression of P-glycoprotein (P-gp), multi-drug resistance-associated protein (MRP1) and glutathione-S-transferase Π (GST-Π) in A549/DDP cells. At the same time, we also established downregulation of p-Akt and decreased transcriptional activation of NF-κB and twist after vinorelbine treatment. The results indicated that vinorelbine might be used as a potential therapeutic strategy in human lung cancer.

• Authors: Yu-Ting Zhou, Kun Li, Hui Tian
• Journal: Asian Pacific journal of cancer prevention : APJCP (Asian Pac J Cancer Prev) Vol. 14 Issue 8 Pg. 4635-9 ( 2013) ISSN: 2476-762X [Electronic] Thailand
• PMID: 24083716 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• ATP Binding Cassette Transporter, Subfamily B, Member 1
• Antineoplastic Agents
• Antineoplastic Agents, Phytogenic
• NF-kappa B
• RNA, Messenger
• Vinblastine
• GSTP1 protein, human
• Glutathione S-Transferase pi
• Proto-Oncogene Proteins c-akt
• Glutathione
• Curcumin
• Vinorelbine

Topics

• ATP Binding Cassette Transporter, Subfamily B, Member 1 (genetics, metabolism)
• Adenocarcinoma (drug therapy, metabolism, pathology)
• Antineoplastic Agents (pharmacology)
• Antineoplastic Agents, Phytogenic (pharmacology)
• Blotting, Western
• Cell Proliferation (drug effects)
• Curcumin (pharmacology)
• Drug Resistance, Multiple (drug effects)
• Drug Resistance, Neoplasm (drug effects)
• Glutathione (metabolism)
• Glutathione S-Transferase pi (genetics, metabolism)
• Humans
• Lung Neoplasms (drug therapy, metabolism, pathology)
• NF-kappa B (genetics, metabolism)
• Phosphorylation (drug effects)
• Proto-Oncogene Proteins c-akt (genetics, metabolism)
• RNA, Messenger (genetics)
• Real-Time Polymerase Chain Reaction
• Reverse Transcriptase Polymerase Chain Reaction
• Tumor Cells, Cultured
• Vinblastine (analogs & derivatives, pharmacology)
• Vinorelbine