Abstract | PURPOSE: MATERIAL AND METHODS: Twenty-six IGM patients were evaluated. Serum samples were analyzed for autoantibodies by indirect immunofluorescence (IIF) using a substrate kit that induced fluorescein-conjugated goat antibodies to human immunoglobulin G ( IgG). IIF patterns were read at serum dilutions of 1 : 40 and 1 : 100 for ANA positivity. Using the immunoblot technique, the sera of patients were assayed at dilutions of 1 : 40 and 1 : 100 for human autoantibodies of the IgG class to 15 lines of highly purified ENAs. RESULTS: In the IIF studies for ANA, positivity was identified for four different patterns in the 1 : 40 diluted preparations, for three different patients in the 1 : 100 diluted preparations and only one pattern was identified at the 1 : 320 dilution. In the ENA studies, positivity was identified for four different pattern in the 1 : 40 dilution, and only one pattern was identified at the 1 : 100 dilution. CONCLUSION: This study was not able to support the eventual existence of an autoimmune basis for IGM.
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Authors | Fatih Altintoprak, Engin Karakece, Taner Kivilcim, Enis Dikicier, Guner Cakmak, Fehmi Celebi, Ihsan Hakkı Ciftci |
Journal | TheScientificWorldJournal
(ScientificWorldJournal)
Vol. 2013
Pg. 148727
( 2013)
ISSN: 1537-744X [Electronic] United States |
PMID | 24082849
(Publication Type: Journal Article)
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Chemical References |
- Antibodies, Antinuclear
- Antigens, Nuclear
- Autoantibodies
- Immunoglobulin G
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Topics |
- Adult
- Aged
- Antibodies, Antinuclear
(metabolism)
- Antigens, Nuclear
(metabolism)
- Autoantibodies
(immunology)
- Autoimmune Diseases
(immunology)
- Female
- Fluorescent Antibody Technique, Indirect
- Granulomatous Mastitis
(immunology)
- Humans
- Immunoblotting
- Immunoglobulin G
(immunology)
- Middle Aged
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