HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Sensitive capture of circulating tumour cells by functionalized graphene oxide nanosheets.

Abstract
The spread of cancer throughout the body is driven by circulating tumour cells (CTCs). These cells detach from the primary tumour and move from the bloodstream to a new site of subsequent tumour growth. They also carry information about the primary tumour and have the potential to be valuable biomarkers for disease diagnosis and progression, and for the molecular characterization of certain biological properties of the tumour. However, the limited sensitivity and specificity of current methods for measuring and studying these cells in patient blood samples prevents the realization of their full clinical potential. The use of microfluidic devices is a promising method for isolating CTCs. However, the devices are reliant on three-dimensional structures, which limits further characterization and expansion of cells on the chip. Here we demonstrate an effective approach to isolating CTCs from blood samples of pancreatic, breast and lung cancer patients, by using functionalized graphene oxide nanosheets on a patterned gold surface. CTCs were captured with high sensitivity at a low concentration of target cells (73 ± 32.4% at 3-5 cells per ml blood).
AuthorsHyeun Joong Yoon, Tae Hyun Kim, Zhuo Zhang, Ebrahim Azizi, Trinh M Pham, Costanza Paoletti, Jules Lin, Nithya Ramnath, Max S Wicha, Daniel F Hayes, Diane M Simeone, Sunitha Nagrath
JournalNature nanotechnology (Nat Nanotechnol) Vol. 8 Issue 10 Pg. 735-41 (Oct 2013) ISSN: 1748-3395 [Electronic] England
PMID24077027 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
Chemical References
  • Biomarkers, Tumor
  • Graphite
Topics
  • Biomarkers, Tumor (blood)
  • Breast Neoplasms (blood, pathology)
  • Cell Line, Tumor
  • Cell Separation (methods)
  • Female
  • Graphite (chemistry)
  • Humans
  • Lung Neoplasms (blood, pathology)
  • MCF-7 Cells
  • Microfluidic Analytical Techniques
  • Nanostructures (chemistry)
  • Neoplastic Cells, Circulating
  • Pancreatic Neoplasms (blood, pathology)
  • Sensitivity and Specificity

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: