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Functional characteristics of connective tissue growth factor on human tenon's capsule fibroblast.

AbstractPURPOSE OF THE STUDY:
To explore the characteristic of connective tissue growth factor (CTGF) on the phenotype transition, extracellular matrix (ECM) synthesis and proliferation of human Tenon's capsule fibroblasts (HTFs).
MATERIALS AND METHODS:
HTFs were obtained from patients during cataract surgery and induced by CTGF (1 to 100 µg/L). Western blot and immunofluorescence were performed to observe the expression of alpha smooth muscle actin (α-SM-actin) protein. The levels of mRNAs were quantified by real-time PCR. Col I and FN expression at both protein and RNA levels were tested after induction by CTGF and transforming growth factor β (TGF-β), respectively. Statistical significance was assumed if p < 0.05.
RESULTS:
CTGF upregulated the expression of α-SM-actin in cultured HTFs. Its maximum effect at protein level attained under the optimal concentration of 50 μg/L at the peak time of 48 hours, though still weaker than the effect of TGF-β1 (10 μg/L, p < 0.05). The expression of Col I and FN at both protein and mRNA levels was elevated by the induction of CTGF (50 μg/L) (p < 0.01) and TGF-β1 (10 μg/L) (p < 0.05), while CTGF (50 μg/L) showed a greater effect than the latter (p < 0.05). CTGF (1 to100 μg/L) increased the proliferation of HTFs significantly (p < 0.05).
CONCLUSIONS:
CTGF induced the phenotype transition of HTFs individually and significantly promoted their proliferation. Moreover, it promoted ECM synthesis, thus demonstrating its role as a crucial factor in fibrosis. Thus, CTGF could potentially be a safer and more efficient target than TGF-β at suppressing scar formation after filtering surgery.
AuthorsJia-Ying Zhang, Peng Gao, Wen Ye, Yi-Qin Xiao
JournalCurrent eye research (Curr Eye Res) Vol. 39 Issue 1 Pg. 53-61 (Jan 2014) ISSN: 1460-2202 [Electronic] England
PMID24074434 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Actins
  • CCN2 protein, human
  • Collagen Type I
  • Fibronectins
  • RNA, Messenger
  • Transforming Growth Factor beta
  • Connective Tissue Growth Factor
Topics
  • Actins (genetics, metabolism)
  • Aged
  • Cell Differentiation (drug effects, physiology)
  • Cells, Cultured
  • Collagen Type I (genetics, metabolism)
  • Connective Tissue Growth Factor (pharmacology, physiology)
  • Extracellular Matrix (drug effects, physiology)
  • Female
  • Fibroblasts (cytology, drug effects)
  • Fibronectins (genetics, metabolism)
  • Gene Expression (drug effects, physiology)
  • Humans
  • Male
  • Middle Aged
  • Myofibroblasts (cytology, drug effects)
  • Phenotype
  • RNA, Messenger (metabolism)
  • Tenon Capsule (cytology, drug effects)
  • Transforming Growth Factor beta (pharmacology, physiology)

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