64Cu-DOTA-trastuzumab PET imaging in patients with HER2-positive breast cancer.

Abstract

UNLABELLED:

The purpose of this study was to determine the safety, distribution, internal dosimetry, and initial human epidermal growth factor receptor 2 (HER2)-positive tumor images of (64)Cu-DOTA-trastuzumab in humans.

METHODS:

PET was performed on 6 patients with primary or metastatic HER2-positive breast cancer at 1, 24, and 48 h after injection of approximately 130 MBq of the probe (64)Cu-DOTA-trastuzumab. Radioactivity data were collected from the blood, urine, and normal-tissue samples of these 6 patients, and the multiorgan biodistribution and internal dosimetry of the probe were evaluated. Safety data were collected for all the patients after the administration of (64)Cu-DOTA-trastuzumab and during the 1-wk follow-up period.

RESULTS:

According to our results, the best timing for the assessment of (64)Cu-DOTA-trastuzumab uptake by the tumor was 48 h after injection. Radiation exposure during (64)Cu-DOTA-trastuzumab PET was equivalent to that during conventional (18)F-FDG PET. The radioactivity in the blood was high, but uptake of (64)Cu-DOTA-trastuzumab in normal tissues was low. In 2 patients, (64)Cu-DOTA-trastuzumab PET showed brain metastases, indicative of blood-brain barrier disruptions. In 3 patients, (64)Cu-DOTA-trastuzumab PET imaging also revealed primary breast tumors at the lesion sites initially identified by CT.

CONCLUSION:

The findings of this study indicated that (64)Cu-DOTA-trastuzumab PET is feasible for the identification of HER2-positive lesions in patients with primary and metastatic breast cancer. The dosimetry and pharmacologic safety results were acceptable at the dose required for adequate PET imaging.

• Authors: Kenji Tamura, Hiroaki Kurihara, Kan Yonemori, Hitoshi Tsuda, Junko Suzuki, Yuzuru Kono, Natsuki Honda, Makoto Kodaira, Harukaze Yamamoto, Mayu Yunokawa, Chikako Shimizu, Koki Hasegawa, Yousuke Kanayama, Satoshi Nozaki, Takayuki Kinoshita, Yasuhiro Wada, Shusaku Tazawa, Kazuhiro Takahashi, Yasuyoshi Watanabe, Yasuhiro Fujiwara
• Journal: Journal of nuclear medicine : official publication, Society of Nuclear Medicine (J Nucl Med) Vol. 54 Issue 11 Pg. 1869-75 (Nov 2013) ISSN: 1535-5667 [Electronic] United States
• PMID: 24029656 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 64Cu-DOTA-trastuzumab
• Antibodies, Monoclonal, Humanized
• Organometallic Compounds
• Receptor, ErbB-2
• Trastuzumab

Topics

• Adult
• Aged
• Antibodies, Monoclonal, Humanized (adverse effects)
• Breast Neoplasms (diagnostic imaging, metabolism, pathology)
• Feasibility Studies
• Humans
• Image Processing, Computer-Assisted
• Middle Aged
• Neoplasm Metastasis
• Organometallic Compounds (adverse effects)
• Positron-Emission Tomography (adverse effects, methods)
• Receptor, ErbB-2 (metabolism)
• Safety
• Trastuzumab