Pseudomonas aeruginosa is an important opportunistic human pathogen that causes severe
infections in immunocompromised patients and also in
cystic fibrosis patients. The aim of this work was to study if a
bovine serum albumin nanoparticles with entrapped
antigens extracted from P. aeruginosa would be able to protect mice from nasal
infection by this pathogen. Mice were immunized via the subcutaneous route using P. aeruginosa
antigens, empty nanoparticles or nanoparticles with entrapped P. aeruginosa
antigens on days 0, 7 and 14. The total
IgG antibody production and specific
IgG1 and
IgG2a titer were measured by ELISA. Immunized mice were challenged with live P. aeruginosa and their lungs were collected for histopathology studies. Our data showed that
NPPa-vaccinated mice presented a high anti-Pseudomonas
IgG1 and a low
IgG2a antibody titles and decreased inflammatory signs, with significant reduction in intensity and concentration of inflammatory cells, lower hemorrhagic,
edema and
hyperemia signs in the lungs of challenge mice with live P. aeruginosa if compared to the other groups. Therefore, this formulation is able to induce a functional response in an animal model of
infection and thereby is a promising platform for P. aeruginosa
vaccines.