Prospective multicentre evaluation of the direct nitrate reductase assay for the rapid detection of extensively drug-resistant tuberculosis.

Abstract

OBJECTIVES:

To perform a multicentre study evaluating the performance of the direct nitrate reductase assay (NRA) for the detection of multidrug-resistant (MDR) and extensively drug-resistant (XDR) tuberculosis in sputum samples.

METHODS:

The study was conducted in six laboratories performing tuberculosis diagnosis that were located in six different countries. The NRA was performed directly on sputum samples in parallel with the reference method used at each site. Detection of resistance was performed for rifampicin, isoniazid, ofloxacin and kanamycin.

RESULTS:

Excellent agreement was obtained for all drugs tested at the majority of sites. The accuracy was 93.7%-100% for rifampicin, 88.2%-100% for isoniazid, 94.6%-100% for ofloxacin and 100% for kanamycin. The majority of NRA results were available at day 21 for sites 1, 2 and 5. Site 3 had a turnaround time of 13.9 days, at site 4 it was 18.4 days and at site 6 it was 16.2 days. The contamination rate ranged between 2.5% and 12%.

CONCLUSIONS:

Rapid detection of drug resistance by the direct NRA on sputum smear-positive samples was accurate and easy to implement in clinical diagnostic laboratories, making it a good alternative for rapid screening for MDR and XDR tuberculosis.

• Authors: Anandi Martin, Belen Imperiale, Pascaline Ravolonandriana, Ahmet Yilmaz Coban, Alper Akgunes, Aamer Ikram, Luqman Satti, Mathieu Odoun, Pooja Pandey, Manvi Mishra, Dissou Affolabi, Urvashi Singh, Voahangy Rasolofo, Nora Morcillo, Peter Vandamme, Juan Carlos Palomino
• Journal: The Journal of antimicrobial chemotherapy (J Antimicrob Chemother) Vol. 69 Issue 2 Pg. 441-4 (Feb 2014) ISSN: 1460-2091 [Electronic] England
• PMID: 24008825 (Publication Type: Evaluation Study, Journal Article, Multicenter Study, Research Support, Non-U.S. Gov't)

Chemical References

• Nitrate Reductase

Topics

• Extensively Drug-Resistant Tuberculosis (diagnosis, enzymology)
• Humans
• Microbial Sensitivity Tests (standards)
• Nitrate Reductase
• Predictive Value of Tests
• Prospective Studies
• Time Factors