Pharmacological potential of Populus nigra extract as antioxidant, anti-inflammatory, cardiovascular and hepatoprotective agent.

Abstract

OBJECTIVE:

To evaluate antioxidant, anti-inflammatory, hepatoprotective and vasorelaxant activities of Populus nigra flower buds ethanolic extract.

METHODS:

Antioxidant and anti-inflammatory activities of the extract were assessed using respectively the ABTS test and the animal model of carrageenan-induced paw edema. Protection from hepatic toxicity caused by aluminum was examined by histopathologic analysis of liver sections. Vasorelaxant effect was estimated in endothelium-intact and -rubbed rings of porcine coronary arteries precontracted with high concentration of U46619.

RESULTS:

The results showed a moderate antioxidant activity (40%), but potent anti-inflammatory activity (49.9%) on carrageenan-induced mice paw edema, and also as revealed by histopathologic examination, complete protection against AlCl₃-induced hepatic toxicity. Relaxant effects of the same extract on vascular preparation from porcine aorta precontracted with high concentration of U46619 were considerable at 10⁻¹ g/L, and comparable (P>0.05) between endothelium-intact (67.74%, IC₅₀=0.04 mg/mL) and -rubbed (72.72%, IC₅₀=0.075 mg/mL) aortic rings.

CONCLUSIONS:

The extract exerted significant anti-inflammatory, hepatoprotective and vasorelaxant activities, the latter being endothelium-independent believed to be mediated mainly by the ability of components present in the extract to exert antioxidant properties, probably related to an inhibition of Ca²⁺ influx.

• Authors: Nadjet Debbache-Benaida, Dina Atmani-Kilani, Valérie Barbara Schini-Keirth, Nouredine Djebbli, Djebbar Atmani
• Journal: Asian Pacific journal of tropical biomedicine (Asian Pac J Trop Biomed) Vol. 3 Issue 9 Pg. 697-704 (Sep 2013) ISSN: 2221-1691 [Print] China
• PMID: 23998009 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Aluminum Compounds
• Anti-Inflammatory Agents
• Cardiovascular Agents
• Chlorides
• Flavonoids
• Phenols
• Plant Extracts
• Protective Agents
• Tannins
• Nitric Oxide
• Aluminum Chloride
• Carrageenan
• Nitric Oxide Synthase Type III
• Nos3 protein, mouse

Topics

• Aluminum Chloride
• Aluminum Compounds (toxicity)
• Analysis of Variance
• Animals
• Anti-Inflammatory Agents (pharmacology)
• Cardiovascular Agents (pharmacology)
• Carrageenan (toxicity)
• Chemical and Drug Induced Liver Injury (drug therapy, pathology)
• Chlorides (toxicity)
• Edema (chemically induced, drug therapy, physiopathology)
• Female
• Flavonoids (analysis)
• Flowers (chemistry)
• Liver (drug effects, pathology)
• Male
• Mice
• Nitric Oxide (metabolism)
• Nitric Oxide Synthase Type III (metabolism)
• Phenols (analysis)
• Plant Extracts (chemistry, pharmacology)
• Populus (chemistry)
• Protective Agents (pharmacology)
• Tannins (analysis)