Expression of sex hormone receptors in juvenile angiofibromas and antiproliferative effects of receptor modulators.

Abstract

BACKGROUND:

Predilection of juvenile angiofibromas in adolescent boys has prompted the hypothesis of hormone-dependent tumor growth. However, knowledge on expression and function of sex hormone receptors in juvenile angiofibromas is still sparse and inconsistent.

METHODS:

Transcript and protein expression of sex hormone receptors in juvenile angiofibromas was studied by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) and immunohistology/fluorescence. A bromodeoxyuridine assay was used to assess the antiproliferative effects of flutamide (androgen receptor antagonist) and tamoxifen (estrogen receptor modulator).

RESULTS:

Significantly increased transcript levels were observed for androgen receptor, estrogen receptor α, follicle-stimulating hormone receptor, and luteinizing hormone receptor in juvenile angiofibromas versus the stroma of nasal mucosa. Estrogen receptor β and progesterone receptor mRNA levels were low and similar for both tissues. Estrogen receptor α protein was detected in juvenile angiofibroma tumors and mesenchymal cell lines. Flutamide and tamoxifen inhibited proliferation of cultured juvenile angiofibroma mesenchymal cells.

CONCLUSION:

These findings contribute to the understanding of juvenile angiofibroma pathophysiology and offer novel therapeutic options.

• Authors: Bernhard Schick, Julia Dlugaiczyk, Olaf Wendler
• Journal: Head & neck (Head Neck) Vol. 36 Issue 11 Pg. 1596-603 (Nov 2014) ISSN: 1097-0347 [Electronic] United States
• PMID: 23996526 (Publication Type: Journal Article)
• Copyright: © 2014 Wiley Periodicals, Inc.

Chemical References

• Antineoplastic Agents, Hormonal
• RNA, Messenger
• Receptors, Androgen
• Receptors, Estrogen
• Receptors, Progesterone
• Tamoxifen
• Flutamide

Topics

• Adolescent
• Angiofibroma (pathology)
• Antineoplastic Agents, Hormonal (pharmacology)
• Cell Proliferation (drug effects)
• Flutamide (pharmacology)
• Gene Expression Regulation, Neoplastic
• Humans
• Male
• Nasal Mucosa (pathology)
• Nose Neoplasms (pathology)
• RNA, Messenger (analysis)
• Real-Time Polymerase Chain Reaction
• Receptors, Androgen (genetics, metabolism)
• Receptors, Estrogen (genetics, metabolism)
• Receptors, Progesterone (genetics, metabolism)
• Sampling Studies
• Sensitivity and Specificity
• Tamoxifen (pharmacology)
• Tumor Cells, Cultured (drug effects)
• Young Adult