HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Vaccination with plasmid DNA encoding a mutant toxic shock syndrome toxin-1 ameliorates toxin-induced lethal shock in mice.

Abstract
Staphylococcal toxic shock syndrome toxin-1 (TSST-1), a superantigenic toxin produced by Staphylococcus (S.) aureus, is a major cause of septic shock and toxic shock syndrome. To investigate whether vaccination with a plasmid DNA encoding a non-toxic mutant TSST-1 (mTSST-1) can protect mice against wild-type TSST-1-induced lethal shock, the mice were intranasally immunized with the plasmid DNA (named pcDNA-mTSST-1) plus a mucosal adjuvant, a non-toxic mutant labile toxin (mLT). After the immunization, the mice were challenged with TSST-1 and lipopolysaccharide (LPS). The survival rate of mice immunized with pcDNA-mTSST-1 plus mLT was higher than that of the control mice immunized with PBS alone, mLT alone, pcDNA-mTSST-1 alone, or a parent plasmid plus mLT. The titers of interferon-γ (IFN-γ) in the sera of mice immunized with pcDNA-mTSST-1 plus mLT were significantly lower than those of the mLT control mice. Immunization with pcDNA-mTSST-1 plus mLT increased the serum levels of TSST-1-specific antibodies, especially immunoglobulin G1 (IgG1) and IgG2a subclasses. Furthermore, the sera obtained from mice immunized with pcDNA-mTSST-1 plus mLT significantly inhibited the TSST-1-induced secretion of IFN-γ and tumor necrosis factor-α (TNF-α) in murine spleen cells in vitro. These results indicate that immunization with pcDNA-mTSST-1 plus mLT provides protection against the lethal toxic shock of mice induced by wild-type TSST-1. The protective effect could be due to TSST-1-specific neutralizing antibodies as well as the inhibition of IFN-γ and TNF-α secretions. Since TSST-1 is commonly released by invasive S. aureus, the pcDNA-mTSST-1 should be useful in preventing toxin-induced shock resulting from S. aureus infection.
AuthorsMao-Hui Feng, Jing-Chun Cui, Akio Nakane, Dong-Liang Hu
JournalThe Tohoku journal of experimental medicine (Tohoku J Exp Med) Vol. 231 Issue 1 Pg. 1-8 (09 2013) ISSN: 1349-3329 [Electronic] Japan
PMID23985881 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Bacterial Toxins
  • Cytokines
  • Enterotoxins
  • Immune Sera
  • Mutant Proteins
  • Superantigens
  • enterotoxin F, Staphylococcal
  • DNA
Topics
  • Animals
  • Antibody Formation (immunology)
  • Bacterial Toxins (immunology)
  • Cytokines (biosynthesis)
  • DNA (immunology)
  • Disease Resistance (immunology)
  • Enterotoxins (immunology)
  • Immune Sera (immunology)
  • Kinetics
  • Mice
  • Mice, Inbred BALB C
  • Mutant Proteins (immunology)
  • Neutralization Tests
  • Plasmids (immunology)
  • Shock, Septic (immunology, microbiology, prevention & control)
  • Staphylococcal Infections (immunology, microbiology, prevention & control)
  • Staphylococcus aureus (immunology)
  • Superantigens (immunology)
  • Vaccination

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: