Cryptosporidium species from human immunodeficiency-infected patients with chronic diarrhea in Jakarta, Indonesia.

Abstract

PURPOSE:

Cryptosporidium is an opportunistic parasite that manifests as chronic and severe diarrhea in the immune-compromised subject. We investigated the species of Cryptosporidium to understand the epidemiology, mode of transmission, response to treatment, and prevention.

METHODS:

Polymerase chain reaction/restriction fragment length polymorphism of the 18 S rRNA gene and sequencing were performed on 41 Cryptosporidium-positive stools from 36 patients with HIV AIDS, which comprised 36 pretreatment stools and 5 stools after treatment with Paromomycin.

RESULTS:

C. hominis, C. meleagridis, C. felis, and C. parvum were detected; 28 of 36 (77.7%) patients were infected with C. hominis and two (5.5%) patients with multiple species of Cryptosporidium. Treatment with Paromomycin resulted in different outcomes, perhaps because patients harbored other intestinal parasitic infections.

CONCLUSIONS:

Multiple infection with various Cryptosporidium species in the presence of other intestinal parasites occurs in patients with HIV AIDS suffering from chronic diarrhea who are severely immune-compromised. Common transmission of Cryptosporidium is anthroponotic.

• Authors: Agnes Kurniawan, Sri W Dwintasari, Lisa Connelly, Rosely A B Nichols, Evy Yunihastuti, Teguh Karyadi, Samsuridjal Djauzi
• Journal: Annals of epidemiology (Ann Epidemiol) Vol. 23 Issue 11 Pg. 720-3 (Nov 2013) ISSN: 1873-2585 [Electronic] United States
• PMID: 23972618 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier Inc. All rights reserved.

Chemical References

• Anti-Bacterial Agents
• Paromomycin

Topics

• AIDS-Related Opportunistic Infections (drug therapy, epidemiology, parasitology)
• Adolescent
• Adult
• Animals
• Anti-Bacterial Agents (therapeutic use)
• Child
• Child, Preschool
• Cross-Sectional Studies
• Cryptosporidiosis (drug therapy, epidemiology, parasitology, transmission)
• Cryptosporidium (classification, genetics, isolation & purification)
• Diarrhea (complications, diagnosis, epidemiology)
• Feces (parasitology)
• Female
• Genes, rRNA
• HIV Infections (complications, epidemiology)
• Humans
• Indonesia (epidemiology)
• Middle Aged
• Paromomycin (therapeutic use)
• Polymerase Chain Reaction
• Polymorphism, Restriction Fragment Length
• Sequence Analysis
• Treatment Outcome
• Young Adult