Lipoxin a4 preconditioning and postconditioning protect myocardial ischemia/reperfusion injury in rats.

Abstract

This study aims to investigate the pre- and postconditioning effects of lipoxin A4 (LXA4) on myocardial damage caused by ischemia/reperfusion (I/R) injury. Seventy-two rats were divided into 6 groups: sham groups (C1 and C2), I/R groups (I/R1 and I/R2), and I/R plus LXA4 preconditioning and postconditioning groups (LX1 and LX2). The serum levels of IL-1 β , IL-6, IL-8, IL-10, TNF- α , and cardiac troponin I (cTnI) were measured. The content and the activity of Na(+)-K(+)-ATPase as well as the superoxide dismutase (SOD), and malondialdehyde (MDA) levels were determined. Along with the examination of myocardium ultrastructure and ventricular arrhythmia scores (VAS), connexin 43 (Cx43) expression were also detected. Lower levels of IL-1 β , IL-6, IL-8, TNF- α , cTnI, MDA content, and VAS and higher levels of IL-10, SOD activity, Na(+)-K(+)-ATPase content and activity, and Cx43 expression appeared in LX groups than I/R groups. Besides, H&E staining, TEM examination as well as analysis of gene, and protein confirmed that LXA4 preconditioning was more effective than postconditioning in preventing arrhythmogenesis via the upregulation of Cx43. That is, LXA4 postconditioning had better protective effect on Na(+)-K(+)-ATPase and myocardial ultrastructure.

• Authors: Qifeng Zhao, Lan Shao, Xingti Hu, Guowei Wu, Jie Du, Jie Xia, Huixian Qiu
• Journal: Mediators of inflammation (Mediators Inflamm) Vol. 2013 Pg. 231351 ( 2013) ISSN: 1466-1861 [Electronic] United States
• PMID: 23956501 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Connexin 43
• Cytokines
• Lipoxins
• Troponin I
• lipoxin A4
• Malondialdehyde
• Superoxide Dismutase
• Sodium-Potassium-Exchanging ATPase

Topics

• Animals
• Arrhythmias, Cardiac (metabolism, prevention & control)
• Connexin 43 (metabolism)
• Cytokines (metabolism)
• Electrocardiography
• Gene Expression Regulation
• Inflammation
• Ischemic Postconditioning
• Ischemic Preconditioning
• Lipoxins (pharmacology)
• Male
• Malondialdehyde (metabolism)
• Microscopy, Electron, Transmission
• Myocardial Reperfusion Injury (pathology, prevention & control)
• Myocardium (pathology, ultrastructure)
• Oxidative Stress
• Rats
• Rats, Sprague-Dawley
• Sodium-Potassium-Exchanging ATPase (metabolism)
• Superoxide Dismutase (metabolism)
• Troponin I (metabolism)