Chronic allograft nephropathy in rats is improved by the intervention of rhein.

Abstract

AIM:

In this study, we investigated the therapeutic efficacy and potential mechanisms of rhein to mitigate chronic allograft nephropathy (CAN) in rats.

MATERIALS AND METHODS:

Fisher rat donors and Lewis rat recipients were used to establish the CAN model. Thirty rats with transplanted kidneys were randomly divided into two groups: 16 untreated and 14 rhein = treated rats. Five Lewis rat controls underwent removal of their right kidneys. The Intervention group was administered rhein oral solution (100 mg kg(-1) d(-1)) by gavage after transplantation. The untreated and control groups were given 0.5% sodium carboxymethyl cellulose. Blood and urine samples were collected at 4, 8, and 16 weeks to examine renal function and total urine protein. Half of the rats in each group were sacrifice at 8 or 16 weeks to examine renal pathology. Immunohistochemical examination and real-time polymerase chain reaction of renal tissues were performed to detect expressions of transforming growth-β1(TGF-β1), hepatic growth factor (HGF), bone morphogenetic protein 7 (BMP7), frobronectin, and collgen IV.

RESULTS:

Rhein improved renal function and significantly reduced renal fibrosis and interstitial inflammation. The levels of BMP7 and HGF were significantly elevated in the renal tissues of the rhein intervention group. In the meantime, fibronectin and collagen IV were decreased in the extracellular matrix. The expression of TGF-β1 was similar between these two groups.

CONCLUSION:

Rhein improved renal function and reduced renal fibrosis and interstitial inflammation by inducing production of HGF and BMP7.

• Authors: J Su, L P Yin, X Zhang, B B Li, L Liu, H Li
• Journal: Transplantation proceedings (Transplant Proc) 2013 Jul-Aug Vol. 45 Issue 6 Pg. 2546-52 ISSN: 1873-2623 [Electronic] United States
• PMID: 23953579 (Publication Type: Journal Article)
• Copyright: Copyright © 2013 Elsevier Inc. All rights reserved.

Chemical References

• Anthraquinones
• Bmp7 protein, rat
• Bone Morphogenetic Protein 7
• Collagen Type IV
• Fibronectins
• RNA, Messenger
• Tgfb1 protein, rat
• Transforming Growth Factor beta1
• Hepatocyte Growth Factor
• rhein

Topics

• Administration, Oral
• Allografts
• Animals
• Anthraquinones (administration & dosage, pharmacology)
• Bone Morphogenetic Protein 7 (genetics, metabolism)
• Collagen Type IV (genetics, metabolism)
• Disease Models, Animal
• Fibronectins (genetics, metabolism)
• Fibrosis
• Gene Expression Regulation
• Glomerulonephritis (genetics, metabolism, pathology, physiopathology, prevention & control)
• Hepatocyte Growth Factor (genetics, metabolism)
• Kidney (drug effects, metabolism, pathology, physiopathology, surgery)
• Kidney Transplantation (adverse effects)
• Male
• Proteinuria (etiology, prevention & control)
• RNA, Messenger (metabolism)
• Rats
• Rats, Inbred F344
• Rats, Inbred Lew
• Renal Insufficiency, Chronic (genetics, metabolism, pathology, physiopathology, prevention & control)
• Time Factors
• Transforming Growth Factor beta1 (genetics, metabolism)