Abstract | RATIONALE: To try to find a correlation between the antiproliferative activity of a series of [M(I)(P)4](+) complexes (M = Cu, Ag and Au; P = tertiary phosphine) and their stability at micromolar concentration under mass spectrometric conditions. METHODS: [M(I)(P)4](+) complexes were investigated by positive ion electrospray ionization mass spectrometry with multiple collisional experiments using an ion trap mass spectrometer. RESULTS: The displacement of P from native [M(I)(P)4](+), previously described for the copper derivative, is common for the triad complexes leading to the formation of [M(P)3](+) and [M( P)2](+) adducts. Further dissociation of [M( P)2](+) depends on the nature of the metal (Cu ~ Ag > Au). More labile [Cu( P)2](+) and [Ag( P)2](+) are more cytotoxic against HCT-15 human colon carcinoma cells compared to less labile [Au( P)2](+) species. CONCLUSIONS: The dissociation of P ligand(s) from the [M(I)(P)4](+) complexes is the driving force for the triggering of the antiproliferative activity. The more favored is the displacement of P from the [M( P)2](+) active form, the more favored is in turn the possibility for the metal to interact with biological substrates related to cancer proliferation.
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Authors | Francesco Tisato, Laura Crociani, Marina Porchia, Plinio Di Bernardo, Francesco Endrizzi, Carlo Santini, Roberta Seraglia |
Journal | Rapid communications in mass spectrometry : RCM
(Rapid Commun Mass Spectrom)
Vol. 27
Issue 17
Pg. 2019-27
(Sep 15 2013)
ISSN: 1097-0231 [Electronic] England |
PMID | 23939970
(Publication Type: Journal Article)
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Copyright | Copyright © 2013 John Wiley & Sons, Ltd. |
Chemical References |
- Phosphines
- Silver
- Gold
- Copper
- phosphine
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Topics |
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Copper
(chemistry, toxicity)
- Gold
(chemistry, toxicity)
- Humans
- Phosphines
(chemistry, toxicity)
- Silver
(chemistry, toxicity)
- Spectrometry, Mass, Electrospray Ionization
- Structure-Activity Relationship
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