Abstract |
Double-negative T ( DNT) cells are αβTCR(+)CD3(+)CD4(-)CD8(-)NK1.1(-) cells that constitute a small but significant proportion of the αβTCR(+) T cells. Their developmental pathway and pathological significance remain unclear. In the present study, we utilized chronic in vitro stimulation of CD4(+) T cells to mimic immune hyper-activation of autoimmune lymphoproliferative syndrome and systemic lupus erythematosus, conditions characterized by DNT cells accumulation. After approximately 4-5 rounds of stimulation, the CD3(+)CD4(-) population became apparent. These cells did not express CD8, NK1.1, γδTCR, or B220, exhibited a highly proliferative effector phenotype, and were dependent on T cell receptor (TCR) stimulation for survival. Moreover, CD3(+)CD4(-) cells expressed MHC class II-restricted αβTCR, indicative of their origin from a CD4(+) T cell population. The results presented herein illustrate a novel method of DNT cell generation in vitro and suggest that immune hyper-activation could also be implicated in the genesis of the disease-associated DNT cells in vivo.
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Authors | Inna V Grishkan, Achilles Ntranos, Peter A Calabresi, Anne R Gocke |
Journal | Cellular immunology
(Cell Immunol)
2013 Jul-Aug
Vol. 284
Issue 1-2
Pg. 68-74
ISSN: 1090-2163 [Electronic] Netherlands |
PMID | 23933188
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2013 Elsevier Inc. All rights reserved. |
Chemical References |
- Receptors, Antigen, T-Cell, alpha-beta
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Topics |
- Animals
- Autoimmune Lymphoproliferative Syndrome
(immunology)
- CD4-Positive T-Lymphocytes
(immunology)
- Cell Proliferation
- Down-Regulation
- Female
- Flow Cytometry
- Lupus Erythematosus, Systemic
(immunology)
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Transgenic
- Phenotype
- Receptors, Antigen, T-Cell, alpha-beta
(immunology)
- T-Lymphocytes, Helper-Inducer
(immunology)
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