Comparison of Fenestra LC, ExiTron nano 6000, and ExiTron nano 12000 for micro-CT imaging of liver and spleen in mice.

Abstract

RATIONALE AND OBJECTIVES:

The purpose of this study was to compare different contrast agents for longitudinal liver and spleen imaging in a mouse model of liver metastasis.

MATERIALS AND METHODS:

Mice developing liver metastases underwent longitudinal micro-computed tomography imaging after injection of Fenestra LC, ExiTron nano 6000, or ExiTron nano 12000. Elimination times and contrast enhancement of liver and spleen were compared.

RESULTS:

For all contrast agents, liver contrast peaked at approximately 4 hours and spleen contrast at 48 hours postinjection. A single dose of 100 μL of ExiTron nano 6000 or 12000 resulted in longstanding enhancement of liver and spleen tissue for longer than 3 weeks, whereas repeated injections of 400 μL of Fenestra LC were required to retain contrast at acceptable levels and allowed imaging of the liver/spleen for up to 2 and 9 days, respectively.

CONCLUSION:

Both ExiTron nano agents provide longer and stronger contrast enhancement of liver and spleen compared to Fenestra LC, and they do so at a 75% lower injection volume in mice.

• Authors: Hanne Boll, Giovanna Figueiredo, Teresa Fiebig, Stefanie Nittka, Fabian Doyon, Hans Ulrich Kerl, Ingo Nölte, Alex Förster, Martin Kramer, Marc A Brockmann
• Journal: Academic radiology (Acad Radiol) Vol. 20 Issue 9 Pg. 1137-43 (Sep 2013) ISSN: 1878-4046 [Electronic] United States
• PMID: 23931428 (Publication Type: Comparative Study, Journal Article)
• Copyright: Copyright © 2013 AUR. Published by Elsevier Inc. All rights reserved.

Chemical References

• Contrast Media

Topics

• Animals
• Cell Line, Tumor
• Contrast Media
• Image Enhancement (methods)
• Liver (diagnostic imaging)
• Liver Neoplasms (diagnostic imaging, secondary)
• Mice
• Mice, Inbred C57BL
• Reproducibility of Results
• Sensitivity and Specificity
• Spleen (diagnostic imaging)
• Splenic Neoplasms (diagnostic imaging)
• Tomography, X-Ray Computed (methods, veterinary)