Abstract | OBJECTIVES: METHODS: Male Sprague-Dawley rats were exposed to hypoxia (O2 10% ± 5% 8 h per day) for 7 days and treated with APO (80 mg/kg, i.g.) in the last 4 days. The PK and tissue distribution of argirein were monitored by measuring its main metabolite rhein using HPLC analysis. Manganese superoxide dismutase (MnSOD) and NOX were assayed. KEY FINDINGS: The PK parameters and concentrations of rhein in the kidney, liver, heart and testes were significantly altered under hypoxia, accompanied with a reduced MnSOD and upregulated NOX compared with the normal. Altered argirein PK and distribution in these organs were relieved following APO administration. CONCLUSION: Abnormal PK and distribution of argirein by assaying its metabolite rhein are significant, consequent to hypoxic injury that is significantly ameliorated by APO through normalizing MITO and NOX.
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Authors | Yu-Mao Zhang, Feng Yu, De-Zai Dai, Jie Gao, Xiao-Dong Cong, Yin Dai |
Journal | The Journal of pharmacy and pharmacology
(J Pharm Pharmacol)
Vol. 65
Issue 9
Pg. 1360-72
(Sep 2013)
ISSN: 2042-7158 [Electronic] England |
PMID | 23927475
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2013 Royal Pharmaceutical Society. |
Chemical References |
- Acetophenones
- Anthraquinones
- Anti-Inflammatory Agents
- Drug Combinations
- Enzyme Inhibitors
- argirein
- Arginine
- acetovanillone
- Superoxide Dismutase
- NADPH Oxidases
- rhein
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Topics |
- Acetophenones
(pharmacology)
- Animals
- Anthraquinones
(metabolism, pharmacokinetics)
- Anti-Inflammatory Agents
(metabolism, pharmacokinetics)
- Arginine
(metabolism, pharmacokinetics)
- Drug Combinations
- Endoplasmic Reticulum Stress
- Enzyme Inhibitors
(pharmacology)
- Hypoxia
(metabolism)
- Male
- Mitochondria
(enzymology, pathology)
- NADPH Oxidases
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Superoxide Dismutase
(metabolism)
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