Astragalus polysaccharide reduces inflammatory response by decreasing permeability of LPS-infected Caco2 cells.

Abstract

As the major constituent of Radix Astragali, Astragalus polysaccharide (APS) is known for its anti-inflammation and immunomodulatory functions. The objective of this study was to investigate the effect of APS on inflammatory response and structural changes in lipopolysaccharide (LPS)-infected Caco2 cells. Caco2 cells were co-cultured with APS and LPS, with APS added after the addition of LPS (post-addition), before the addition of LPS (pre-addition), or simultaneously with the addition of LPS (simultaneous addition). The mRNA expression of inflammatory indicators and tight junctions was measured by RT-qPCR. Short circuit current (Isc) was recorded by an Ussing chamber system. Addition of APS significantly down-regulated the expression of TNF-α, IL-1β and IL-8 (P<0.05) and the Isc levels (P<0.05) of LPS-infected Caco2 cells for all three administration treatments. The minimum anti-inflammatory concentration of APS was 50, 100, and 100 μg/mL for pre-, post-, and simultaneous additions of APS, respectively. The mRNA expression of zonula occludens-1 (ZO-1) and occludin was significantly up-regulated for post- and pre-additions of APS, respectively (P<0.05). Results suggested that APS had anti-inflammatory and structure protective properties for LPS-infected Caco2 cells, and may be used as a preventative treatment for intestine cells.

• Authors: Xiaofei Wang, Yulong Li, Xiaojun Yang, Junhu Yao
• Journal: International journal of biological macromolecules (Int J Biol Macromol) Vol. 61 Pg. 347-52 (Oct 2013) ISSN: 1879-0003 [Electronic] Netherlands
• PMID: 23916649 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier B.V. All rights reserved.

Chemical References

• Anti-Inflammatory Agents
• Lipopolysaccharides
• Occludin
• Polysaccharides
• RNA, Messenger
• Zonula Occludens-1 Protein

Topics

• Anti-Inflammatory Agents (pharmacology, toxicity)
• Astragalus Plant (chemistry)
• Caco-2 Cells
• Gene Expression Regulation (drug effects)
• Humans
• Lipopolysaccharides
• Occludin (genetics, metabolism)
• Permeability (drug effects)
• Polysaccharides (pharmacology, toxicity)
• RNA, Messenger (genetics, metabolism)
• Zonula Occludens-1 Protein (genetics, metabolism)