MiR-125b, miR-100 and miR-99a co-regulate vincristine resistance in childhood acute lymphoblastic leukemia.

Abstract

MicroRNA-125b (miR-125b), miR-99a and miR-100 are overexpressed in vincristine-resistant acute lymphoblastic leukemia (ALL). Cellular viability of ETV6-RUNX1-positive Reh cells significantly increased in presence of 9 ng/mL vincristine upon co-expression of miR-125b/miR-99a (91 ± 4%), miR-125b/miR-100 (93 ± 5%) or miR-125b/miR-99a/miR-100 (82 ± 17%) compared with miR-125b-transduced cells (38 ± 13%, P<0.05). Co-expression of these miRNAs resulted in downregulation of DNTT, NUCKS1, MALAT1, SNRPE, PNO1, SET, KIF5B, PRPS2, RPS11, RPL38 and RPL23A (fold-change 1.3-1.9, p<0.05). Similarly, 7 out of these genes are lower expressed in vincristine-resistant ALL cells of children (p<0.05). The concerted function of miR-125b in combination with miR-99a and/or miR-100 illustrates the complexity of vincristine-resistant pediatric ALL.

• Authors: F Akbari Moqadam, E A M Lange-Turenhout, I M Ariës, R Pieters, M L den Boer
• Journal: Leukemia research (Leuk Res) Vol. 37 Issue 10 Pg. 1315-21 (Oct 2013) ISSN: 1873-5835 [Electronic] England
• PMID: 23915977 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier Ltd. All rights reserved.

Chemical References

• Antineoplastic Agents, Phytogenic
• MIRN100 microRNA, human
• MIRN125 microRNA, human
• MIRN99 microRNA, human
• MicroRNAs
• Vincristine

Topics

• Antineoplastic Agents, Phytogenic (pharmacology, therapeutic use)
• Base Sequence
• Child
• Child, Preschool
• Drug Resistance, Neoplasm (genetics)
• Gene Expression
• Gene Expression Regulation, Leukemic
• Gene Order
• Humans
• MicroRNAs (genetics)
• Precursor Cell Lymphoblastic Leukemia-Lymphoma (drug therapy, genetics)
• Vincristine (pharmacology, therapeutic use)