Inhibition of cell adhesion by a cadherin-11 antibody thwarts bone metastasis.

Abstract

UNLABELLED:

Cadherin-11 (CDH11) is a member of the cadherin superfamily mainly expressed in osteoblasts but not in epithelial cells. However, prostate cancer cells with a propensity for bone metastasis express high levels of cadherin-11 and reduced levels of E-cadherin. Downregulation of cadherin-11 inhibits interaction of prostate cancer cells with osteoblasts in vitro and homing of prostate cancer cells to bone in an animal model of metastasis. These findings indicate that targeting cadherin-11 may prevent prostate cancer bone metastasis. To explore this possibility, a panel of 21 monoclonal antibodies (mAb) was generated against the extracellular (EC) domain of cadherin-11. Two antibodies, mAbs 2C7 and 1A5, inhibited cadherin-11-mediated cell-cell aggregation in vitro using L-cells transfected with cadherin-11. Both antibodies demonstrated specificity to cadherin-11, and neither antibody recognized E-cadherin or N-cadherin on C4-2B or PC3 cells, respectively. Furthermore, mAb 2C7 inhibited cadherin-11-mediated aggregation between the highly metastatic PC3-mm2 cells and MC3T3-E1 osteoblasts. Mechanistically, a series of deletion mutants revealed a unique motif, aa 343-348, in the cadherin-11 EC3 domain that is recognized by mAb 2C7 and that this motif coordinated cell-cell adhesion. Importantly, administration of mAb 2C7 in a prophylactic setting effectively prevented metastasis of PC3-mm2 cells to bone in an in vivo mouse model. These results show that targeting the extracellular domain of cadherin-11 can limit cellular adhesion and metastatic dissemination of prostate cancer cells.

IMPLICATIONS:

Monotherapy using a cadherin-11 antibody is a suitable option for the prevention of bone metastases.

• Authors: Yu-Chen Lee, Mehmet Asim Bilen, Guoyu Yu, Song-Chang Lin, Chih-Fen Huang, Angelica Ortiz, Hyojin Cho, Jian H Song, Robert L Satcher, Jian Kuang, Gary E Gallick, Li-Yuan Yu-Lee, Wilber Huang, Sue-Hwa Lin
• Journal: Molecular cancer research : MCR (Mol Cancer Res) Vol. 11 Issue 11 Pg. 1401-11 (Nov 2013) ISSN: 1557-3125 [Electronic] United States
• PMID: 23913163 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
• Copyright: ©2013 AACR.

Chemical References

• Antibodies, Monoclonal
• Cadherins
• osteoblast cadherin

Topics

• Animals
• Antibodies, Monoclonal (pharmacology)
• Binding Sites, Antibody
• Bone Neoplasms (pathology, secondary)
• Cadherins (genetics, immunology)
• Cell Adhesion
• Cell Movement
• Epitope Mapping
• Humans
• Male
• Mice
• Mice, SCID
• Mutation
• Neoplasm Metastasis
• Prostatic Neoplasms (pathology)