Abstract | BACKGROUND: SCOPE OF REVIEW: We give a critical appraisal of the best methods to measure production of hypochlorous acid by purified peroxidases and isolated neutrophils. Robust ways of detecting it inside neutrophil phagosomes where bacteria are killed are also discussed. Special attention is focused on reaction-based fluorescent probes but their visual charm is tempered by stressing their current limitations. Finally, the strengths and weaknesses of biomarker assays that capture the footprints of chlorine in various pathologies are evaluated. MAJOR CONCLUSIONS: GENERAL SIGNIFICANCE: These methods should bring new insights into how chlorine bleach is produced by peroxidases, reacts within phagosomes to kill bacteria, and contributes to inflammation. This article is part of a Special Issue entitled Current methods to study reactive oxygen species - pros and cons and biophysics of membrane proteins. Guest Editor: Christine Winterbourn.
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Authors | Anthony J Kettle, Amelia M Albrett, Anna L Chapman, Nina Dickerhof, Louisa V Forbes, Irada Khalilova, Rufus Turner |
Journal | Biochimica et biophysica acta
(Biochim Biophys Acta)
Vol. 1840
Issue 2
Pg. 781-93
(Feb 2014)
ISSN: 0006-3002 [Print] Netherlands |
PMID | 23872351
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Review)
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Copyright | © 2013. |
Chemical References |
- Hypochlorous Acid
- Peroxidase
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Topics |
- Animals
- Humans
- Hypochlorous Acid
(analysis)
- Inflammation
(metabolism)
- Neutrophils
(metabolism)
- Peroxidase
(metabolism)
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