A thermostable trypsin inhibitor with antiproliferative activity from small pinto beans.

Abstract

Small pinto bean is a cultivar of Phaseolus vulgaris. It produces a 16-kDa trypsin inhibitor that could be purified using anion exchange and size chromatography. Q-Sepharose, Mono Q and Superdex 75 columns were employed for the isolation process. Small pinto bean trypsin inhibitor demonstrated moderate pH stability (pH 2-10) and marked heat stability, with its trypsin inhibitory activity largely retained after exposure to 100 °C for half an hour. The activity was abolished in the presence of dithiothreitol, in a dose-dependent manner, implying that disulfide bonds in small pinto bean trypsin inhibitor are crucial for the activity. The trypsin inhibitor showed a blocked N-terminus. The trypsin inhibitor only slightly inhibited the viability of breast cancer MCF7 and hepatoma HepG2 cells at 125 μM.

• Authors: Yau Sang Chan, Yanbo Zhang, Stephen Cho Wing Sze, Tzi Bun Ng
• Journal: Journal of enzyme inhibition and medicinal chemistry (J Enzyme Inhib Med Chem) Vol. 29 Issue 4 Pg. 485-90 (Aug 2014) ISSN: 1475-6374 [Electronic] England
• PMID: 23859150 (Publication Type: Journal Article)

Chemical References

• Trypsin Inhibitors
• Trypsin

Topics

• Cell Proliferation (drug effects)
• Cell Survival (drug effects)
• Dose-Response Relationship, Drug
• Drug Screening Assays, Antitumor
• Hep G2 Cells
• Humans
• MCF-7 Cells
• Phaseolus (chemistry)
• Seeds (chemistry)
• Structure-Activity Relationship
• Temperature
• Trypsin (metabolism)
• Trypsin Inhibitors (chemistry, isolation & purification, pharmacology)