Evaluation of the wound healing activity of Shorea robusta, an Indian ethnomedicine, and its isolated constituent(s) in topical formulation.

Different parts of Indian ethnomedicinal plant Shorea robusta is traditionally used for several ailments including wounds and burn by different tribal groups, since ages. Here we have validated, for the first time, the effectiveness and the possible mechanism of action of young leaf extracts of Shorea robusta, used by two distinct tribes of India, and its isolated compounds as a topical formulation in three wound models in rats.
Bioactivity-guided study of the active extract resulted in the isolation of two known compounds. The prepared ointment containing extracts (2.5 and 5%, w/w), fractions (5% w/w) and isolated compounds (0.25% w/w) were evaluated on excision, incision and dead space wound models in rats by the rate of wound closure, period of epithelialisation, tensile strength, granulation tissue weight, hydroxyproline content and histopathology.
The animals treated with the extracts and fractions (5%) showed significant reduction in wound area 96.55 and 96.41% with faster epithelialisation (17.50 and 17.86), while the isolated compounds bergenin and ursolic acid heal the wound faster, but complete epithelialisation with 100% wound contraction was evident with 5% povidone-iodine group on 18th post-wounding day. Moreover, the tensile strength of incision wound, granuloma tissue weight, and hydroxyproline content was significantly increased in both the extract and compound(s) treated animals. Furthermore, the tissue histology of animals treated with the isolated compound(s) showed complete epithelialisation with increased collagenation, similar to povidone-iodine group.
Thus, our results validated the traditional use of Shorea robusta young leaves in wound management.
AuthorsHemanta Mukherjee, Durbadal Ojha, Yogesh P Bharitkar, Soma Ghosh, Supriya Mondal, Sudipta Kaity, Shanta Dutta, Amalesh Samanta, Tapan K Chatterjee, Sekhar Chakrabarti, Nirup B Mondal, Debprasad Chattopadhyay
JournalJournal of ethnopharmacology (J Ethnopharmacol) Vol. 149 Issue 1 Pg. 335-43 (Aug 26 2013) ISSN: 1872-7573 [Electronic] Ireland
PMID23838474 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2013 Elsevier Ireland Ltd. All rights reserved.
Chemical References
  • Plant Extracts
  • Administration, Topical
  • Animals
  • Cell Survival (drug effects)
  • Cells, Cultured
  • Dipterocarpaceae (chemistry)
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • Drug Stability
  • Ethnopharmacology
  • India
  • Lethal Dose 50
  • Macrophages (drug effects, immunology)
  • Mice
  • Plant Extracts (isolation & purification, pharmacology, therapeutic use, toxicity)
  • Plant Leaves (chemistry)
  • Rats
  • Rats, Wistar
  • Skin Irritancy Tests
  • Toxicity Tests, Acute
  • Wound Healing (drug effects)
  • Wounds, Penetrating (drug therapy)

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