Gadolinium-functionalized aggregation-induced emission dots as dual-modality probes for cancer metastasis study.

Abstract

Understanding the localization and engraftment of tumor cells at postintravasation stage of metastasis is of high importance in cancer diagnosis and treatment. Advanced fluorescent probes and facile methodologies for cell tracing play a key role in metastasis studies. In this work, we design and synthesize a dual-modality imaging dots with both optical and magnetic contrast through integration of a magnetic resonance imaging reagent, gadolinium(III), into a novel long-term cell tracing probe with aggregation-induced emission (AIE) in far-red/near-infrared region. The obtained fluorescent-magnetic AIE dots have both high fluorescence quantum yield (25%) and T1 relaxivity (7.91 mM(-1) s(-1) ) in aqueous suspension. After further conjugation with a cell membrane penetrating peptide, the dual-modality dots can be efficiently internalized into living cells. The gadolinium(III) allows accurate quantification of biodistribution of cancer cells via intraveneous injection, while the high fluorescence provides engraftment information of cells at single cellular level. The dual-modality AIE dots show obvious synergistic advantages over either single imaging modality and hold great promises in advanced biomedical studies.

• Authors: Kai Li, Dan Ding, Chandrasekharan Prashant, Wei Qin, Chang-Tong Yang, Ben Zhong Tang, Bin Liu
• Journal: Advanced healthcare materials (Adv Healthc Mater) Vol. 2 Issue 12 Pg. 1600-5 (Dec 2013) ISSN: 2192-2659 [Electronic] Germany
• PMID: 23836611 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Cell-Penetrating Peptides
• Coordination Complexes
• Fluorescent Dyes
• Phosphatidylethanolamines
• Polyethylene Glycols
• Gadolinium

Topics

• Animals
• Cell Line, Tumor
• Cell Tracking (instrumentation, methods)
• Cell-Penetrating Peptides (chemistry, pharmacokinetics)
• Coordination Complexes (chemistry)
• Fluorescent Dyes (chemistry, pharmacokinetics)
• Gadolinium (chemistry, pharmacokinetics)
• Magnetic Resonance Imaging
• Male
• Mice
• Mice, SCID
• Molecular Imaging
• Nanoparticles (chemistry)
• Neoplasm Metastasis (pathology)
• Phosphatidylethanolamines (chemistry)
• Polyethylene Glycols (chemistry)
• Rats
• Tissue Distribution