[The influence of rhAng-1 on endothelial junctions of BBB after focal cerebral ischemia/reperfusion in rats].

Abstract

OBJECTIVE:

This study was performed to determine whether recombinant human angiopoietin-1 (rhAng-1) decreases the permeability of the blood-brain barrier (BBB) in focal cerebral ischemia and reperfusion rats, whether RhAng-1 opens the BBB by affecting tight junction associated proteins zonnula occludin-1 (ZO-1), occludin and adherens junction protein vascular endothelial (VE)-cadherin.

METHODS:

The rats were divided into eight groups randomly( n = 10): (1) sham-operated group; (2) ischemia group; (3)-(5) ischemia/reperfusion (middle cerebral artery occlusion and reperfusion (MCAO/R) 12 h, 48 h, and 7 days) and 0.9% saline groups; (6)-(8) ischemia/reperfusion (MCAO/R 12 h, 48 h, and 7 days) and rhAng-1 groups. The Bee permeability was assessed by Evans blue extravasation. The messenger RNA and protein expressions of ZO-1, occludin, and VE-cadherin were determined by reverse transcription-polymerase chain reaction, Western blot, and immunohistochemistry assays.

RESULTS:

The BBB permeability and the brain infarct volume were significantly decreased after rhAng-1 injection. The expressions of ZO-1, occludin, and VE-cadherin were increased after rhAng-1 injection.

CONCLUSION:

rhAng-1 may decrease the permeability of BBB in MCAO/R rats by upregulation of ZO-1, occludin, and VE-cadherin.

• Authors: Hang Yu, Qing-Bo Jia, Yi-Xue Xue
• Journal: Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology (Zhongguo Ying Yong Sheng Li Xue Za Zhi) Vol. 29 Issue 2 Pg. 147-52 (Mar 2013) ISSN: 1000-6834 [Print] China
• PMID: 23833970 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Angiopoietin-1
• Antigens, CD
• Cadherins
• Occludin
• Ocln protein, rat
• Recombinant Proteins
• Tjp1 protein, rat
• Zonula Occludens-1 Protein
• cadherin 5

Topics

• Angiopoietin-1 (pharmacology)
• Animals
• Antigens, CD (metabolism)
• Blood-Brain Barrier (cytology, metabolism)
• Brain Ischemia (metabolism)
• Cadherins (metabolism)
• Endothelium, Vascular (cytology, drug effects)
• Humans
• Male
• Occludin (metabolism)
• Rats
• Rats, Wistar
• Recombinant Proteins (pharmacology)
• Reperfusion Injury (metabolism)
• Zonula Occludens-1 Protein (metabolism)